HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/6/F1204    most recent 00063.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Song, J. Articles by Ecelbarger, C. A. Search for Related Content PubMed PubMed Citation Articles by Song, J. Articles by Ecelbarger, C. A.

Effects of dietary fat, NaCl, and fructose on renal sodium and water transporter abundances and systemic blood pressure

¹Department of Medicine, Division of Endocrinology and Metabolism, Georgetown University, Washington, District of Columbia 20057; and ²Laboratory of Kidney and Electrolyte Metabolism, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892

Submitted 24 February 2004 ; accepted in final form 4 August 2004

Dietary fructose, NaCl, and/or saturated fat have been correlated with mean arterial pressure (MAP) rises in sensitive strains of rats. Dysregulation of sodium and/or water reabsorption by the kidney may contribute. Using radiotelemetry and parallel semiquantitative immunoblotting, we examined the effects of various diets on MAP and the regulation of abundance of the major renal sodium and water transport proteins in male Sprague-Dawley rats. In study 1, rats (275 g) were fed one of four diets for 4 wk (n = 6/group): 1) control, 2) 65% fructose, 3) control + added NaCl (2.59%), or 4) fructose + NaCl. In study 2, 5% butter (fat) was added to the above four diets. Both fat and NaCl, but not fructose, caused modest rises in MAP (5–10 mmHg) and increased the day-to-night ratio in diastolic blood pressure. NaCl or fructose increased kidney size. Creatinine clearance was increased by salt or fat, and fractional excretion of sodium was decreased by fat. In study 1, high NaCl markedly reduced plasma renin and aldosterone and its regulated proteins in whole kidney, i.e., the thiazide-sensitive Na-Cl cotransporter and the - and (70-kDa band)-subunits of the epithelial sodium channel. These effects were blunted by fat. Fructose increased the abundance of the sodium phosphate cotransporter, whereas it decreased the bumetanide-sensitive Na-K-2Cl cotransporter and aquaporin-2. Overall, doubling of dietary fat appeared to impair dietary sodium adaptation, i.e., blunt the downregulation of aldosterone-mediated effects, thus allowing blood pressure to rise at an accelerated rate.

insulin resistance; natriuresis; diuresis; NaPi-2; sodium hydrogen exchanger 3; BSC1; TSC; epithelial sodium channel

This article has been cited by other articles:

				S. Tiwari, S. Riazi, and C. A. Ecelbarger Insulin's impact on renal sodium transport and blood pressure in health, obesity, and diabetes Am J Physiol Renal Physiol, October 1, 2007; 293(4): F974 - F984. [Abstract] [Full Text] [PDF]

				S. Tiwari, V. K.M. Halagappa, S. Riazi, X. Hu, and C. A. Ecelbarger Reduced Expression of Insulin Receptors in the Kidneys of Insulin-Resistant Rats J. Am. Soc. Nephrol., October 1, 2007; 18(10): 2661 - 2671. [Abstract] [Full Text] [PDF]

				M. B. Sandberg, A. D. M. Riquier, K. Pihakaski-Maunsbach, A. A. McDonough, and A. B. Maunsbach ANG II provokes acute trafficking of distal tubule Na+-Cl cotransporter to apical membrane Am J Physiol Renal Physiol, September 1, 2007; 293(3): F662 - F669. [Abstract] [Full Text] [PDF]

				M. B. Sandberg, A. B. Maunsbach, and A. A. McDonough Redistribution of distal tubule Na+-Cl- cotransporter (NCC) in response to a high-salt diet Am J Physiol Renal Physiol, August 1, 2006; 291(2): F503 - F508. [Abstract] [Full Text] [PDF]

				J. Song, X. Hu, S. Riazi, S. Tiwari, J. B. Wade, and C. A. Ecelbarger Regulation of blood pressure, the epithelial sodium channel (ENaC), and other key renal sodium transporters by chronic insulin infusion in rats Am J Physiol Renal Physiol, May 1, 2006; 290(5): F1055 - F1064. [Abstract] [Full Text] [PDF]

				D. Y. Huang, K. M. Boini, B. Friedrich, M. Metzger, L. Just, H. Osswald, P. Wulff, D. Kuhl, V. Vallon, and F. Lang Blunted hypertensive effect of combined fructose and high-salt diet in gene-targeted mice lacking functional serum- and glucocorticoid-inducible kinase SGK1 Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2006; 290(4): R935 - R944. [Abstract] [Full Text] [PDF]

				O. Khan, S. Riazi, X. Hu, J. Song, J. B. Wade, and C. A. Ecelbarger Regulation of the renal thiazide-sensitive Na-Cl cotransporter, blood pressure, and natriuresis in obese Zucker rats treated with rosiglitazone Am J Physiol Renal Physiol, August 1, 2005; 289(2): F442 - F450. [Abstract] [Full Text] [PDF]