HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 287/6/F1233    most recent 00155.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Nissant, A. Articles by Imbert-Teboul, M. Search for Related Content PubMed PubMed Citation Articles by Nissant, A. Articles by Imbert-Teboul, M.

Heterogeneous distribution of chloride channels along the distal convoluted tubule probed by single-cell RT-PCR and patch clamp

Centre National de la Recherche Scientifique and Université Pierre et Marie Curie, UMR 7134, Institut des Cordeliers, 75270 Paris Cedex 06, France

Submitted 30 April 2004 ; accepted in final form 23 July 2004

The distal convoluted tubule (DCT) is a heterogeneous segment subdivided into early (DCT1) and late (DCT2) parts, depending on the distribution of various transport systems. We do not have an exhaustive picture of the Cl^– channels on the basolateral side: the presence of ClC-K2 channels is generally accepted, whereas that of ClC-K1 remains controversial. We used here single-cell RT-PCR and patch clamp to probe Cl^– channel heterogeneity in microdissected mouse DCT at the molecular and functional levels. Our findings show that 63% of the DCT cells express ClC-K2 mRNA, either alone (type 1 cells: 47 and 23% in DCT1 and DCT2, respectively), or combined with ClC-K1, mostly in DCT2 (type 2 cells: 33%), but 37% of DCT1 and DCT2 cells do not express any ClC-K. Patch-clamp experiments revealed that a Cl^– channel, with 9-pS conductance and Cl^– > NO₃^– = Br^– anion selectivity sequence, is present in the DCT1 and DCT2 basolateral membranes (87 and 71% of the patches, respectively). This dominant channel is likely to be ClC-K2 in type 1 cells. In type 2 cells, it could be ClC-K2 and/or ClC-K1 homodimers, but also ClC-K1/ClC-K2 heterodimers, or a mixture of all combinations. A second, distinct Cl^– channel (13% of DCT1 patches, 29% of DCT2 patches) also displayed 9-pS conductance but had a completely different anion selectivity (I^– > NO₃^– > Br^– > Cl^–), which was not compatible with that of the ClC-Ks. This indicates that a Cl^– channel that is unlikely to belong to the ClC family may also be involved in Cl^– absorption in the DCT2.

kidney; ClC-K; sodium chloride absorption

This article has been cited by other articles:

				G. Capasso, M. Rizzo, M. L. Garavaglia, F. Trepiccione, M. Zacchia, A. Mugione, P. Ferrari, M. Paulmichl, F. Lang, J. Loffing, et al. Upregulation of apical sodium-chloride cotransporter and basolateral chloride channels is responsible for the maintenance of salt-sensitive hypertension Am J Physiol Renal Physiol, August 1, 2008; 295(2): F556 - F567. [Abstract] [Full Text] [PDF]

				S. Lachheb, F. Cluzeaud, M. Bens, M. Genete, H. Hibino, S. Lourdel, Y. Kurachi, A. Vandewalle, J. Teulon, and M. Paulais Kir4.1/Kir5.1 channel forms the major K+ channel in the basolateral membrane of mouse renal collecting duct principal cells Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1398 - F1407. [Abstract] [Full Text] [PDF]

				L. G. Palmer and G. Frindt Cl- channels of the distal nephron Am J Physiol Renal Physiol, December 1, 2006; 291(6): F1157 - F1168. [Abstract] [Full Text] [PDF]

				A. Nissant, M. Paulais, S. Lachheb, S. Lourdel, and J. Teulon Similar chloride channels in the connecting tubule and cortical collecting duct of the mouse kidney Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1421 - F1429. [Abstract] [Full Text] [PDF]

				M. Paulais, S. Lachheb, and J. Teulon A Na+- and Cl--activated K+ Channel in the Thick Ascending Limb of Mouse Kidney J. Gen. Physiol., January 30, 2006; 127(2): 205 - 215. [Abstract] [Full Text] [PDF]

				T. J. Jentsch Chloride Transport in the Kidney: Lessons from Human Disease and Knockout Mice J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1549 - 1561. [Abstract] [Full Text] [PDF]