HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 288/2/F265    most recent 00318.2004v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Agarwal, R. Search for Related Content PubMed PubMed Citation Articles by Agarwal, R.

TRANSLATIONAL PHYSIOLOGY

On the nature of proteinuria with acute renal injury in patients with chronic kidney disease

Department of Medicine, Division of Nephrology, Indiana University School of Medicine, and Richard L. Roudebush Veterans Affairs Medical Center, Indianapolis, Indiana

Albuminuria is an excellent marker of cardiovascular and renal prognosis. Commercially available tests of immunodetectable albumin in the urine may not identify posttranslationally modified albumin that makes it undetectable to antibodies. Also, it is unclear whether albumin is degraded to smaller fragments, such as through proteolysis, in the course of acute renal injury. In 20 men with chronic kidney disease, we measured excretion rates of urinary protein (pyragallol red), immundetectable urinary albumin (immunoturbidimetry), and urinary total intact albumin (HPLC) after a single dose of 100 mg intravenous iron sucrose administered over 5 min. Fragmentation of urinary albumin and carbonylation of urinary proteins were assessed by immunoblotting. Results showed that iron infusion increased carbonylation of plasma and urinary proteins in a time-dependent manner. A transient increase in urinary excretion rates of total protein, immunodetectable urinary albumin, and total intact albumin was seen. Fragmentation and loss of immunoreactivity of albumin paralleled the changes in total protein excretion. In conclusion, fragmentation, loss of immunoreactivity, and oxidation of albumin in a time-dependent manner may underestimate the extent of injury with the immunoreactive microalbumin assay. Measurement of total intact albumin may better quantify acute renal injury.

oxidative stress; albuminuria

This article has been cited by other articles:

				D. J. Birmingham, B. H. Rovin, G. Shidham, M. Bissell, H. N. Nagaraja, and L. A. Hebert Relationship between Albuminuria and Total Proteinuria in Systemic Lupus Erythematosus Nephritis: Diagnostic and Therapeutic Implications Clin. J. Am. Soc. Nephrol., July 1, 2008; 3(4): 1028 - 1033. [Abstract] [Full Text] [PDF]

				B. L. West, M. M. Picken, and D. J. Leehey Albuminuria in acute tubular necrosis Nephrol. Dial. Transplant., October 1, 2006; 21(10): 2953 - 2956. [Full Text] [PDF]

				K. Bishu and R. Agarwal Acute Injury with Intravenous Iron and Concerns Regarding Long-Term Safety Clin. J. Am. Soc. Nephrol., September 1, 2006; 1(Supplement_1): S19 - S23. [Abstract] [Full Text] [PDF]