HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 288/6/F1164    most recent 00199.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (11) Citing Articles via Google Scholar Google Scholar Articles by Kishore, B. K. Articles by Nelson, R. D. Search for Related Content PubMed PubMed Citation Articles by Kishore, B. K. Articles by Nelson, R. D.

P2Y2 receptor mRNA and protein expression is altered in inner medullas of hydrated and dehydrated rats: relevance to AVP-independent regulation of IMCD function

Departments of ¹Medicine and ²Physiology, and ⁴Division of Pediatric Nephrology, University of Utah Health Sciences Center, Salt Lake City; ³Nephrology Research, Veterans Affairs Salt Lake City Health Care System, Salt Lake City, Utah; and ⁵Department of Biology, University of Dayton, Dayton, Ohio

Submitted 4 June 2004 ; accepted in final form 31 January 2005

Arginine vasopressin (AVP), acting through a cAMP second messenger system, regulates osmotic water permeability (P_f) of the collecting duct. In the collecting duct, the activities of cAMP and phosphonositides (PI) are mutually inhibitory. The P2Y2 receptor (P2Y2-R) is a G protein-coupled extracellular nucleotide receptor associated with PI signaling pathway. Previously, we showed that P2Y2-R is expressed in inner medullary collecting duct (IMCD) of rat, and its agonist (ATP/UTP) activation decreased AVP-induced P_f and resulted in enhanced production of prostaglandin E₂. Hydrated and dehydrated states are associated with alterations in the circulating levels of AVP, expression and/or subcellular distribution of AVP-regulated aquaporin-2 water channel in IMCD and thus P_f of IMCD. We hypothesized that altered expression and/or signaling via P2Y2-R may also modulate IMCD function in these conditions. Sprague-Dawley rats were subjected to dehydration by water deprivation (48 h) or hydration (48 or 96 h) by providing sucrose water. Hydration or dehydration resulted in marked alterations in mRNA expression (Northern blot analysis and real-time RT-PCR) and protein abundance (Western blot analysis) of P2Y2-R, with hydrated rats showing significantly higher levels compared with dehydrated rats. Sequential hydration and dehydration experiments also revealed that the regulated expression profiles of P2Y2-R mRNA and protein are discordant. Conversely, the expression of V2-R mRNA remained unaltered during hydration and dehydration. Because virtually all renal cells release ATP in a regulated fashion, the observed alterations in P2Y2-R expression in the inner medulla in hydrated and dehydrated states may constitute a novel mechanism of purinergic modulation of IMCD function.

purinergic; V2; cAMP; prostanoids; nucleotides

This article has been cited by other articles:

				T. Rieg, K. Pothula, J. Schroth, J. Satriano, H. Osswald, J. Schnermann, P. A. Insel, R. A. Bundey, and V. Vallon Vasopressin regulation of inner medullary collecting ducts and compensatory changes in mice lacking adenosine A1 receptors Am J Physiol Renal Physiol, March 1, 2008; 294(3): F638 - F644. [Abstract] [Full Text] [PDF]

				V. Vallon P2 receptors in the regulation of renal transport mechanisms Am J Physiol Renal Physiol, January 1, 2008; 294(1): F10 - F27. [Abstract] [Full Text] [PDF]

				T. Rieg, R. A. Bundey, Y. Chen, G. Deschenes, W. Junger, P. A. Insel, and V. Vallon Mice lacking P2Y2 receptors have salt-resistant hypertension and facilitated renal Na+ and water reabsorption FASEB J, November 1, 2007; 21(13): 3717 - 3726. [Abstract] [Full Text] [PDF]

				B. K. Kishore, J. Isaac, and C. Westenfelder Administration of poly-D-glutamic acid induces proliferation of erythropoietin-producing peritubular cells in rat kidney Am J Physiol Renal Physiol, February 1, 2007; 292(2): F749 - F761. [Abstract] [Full Text] [PDF]

				E. Cristia, C. Amat, R. J. Naftalin, and M. Moreto Role of vasopressin in rat distal colon function J. Physiol., January 15, 2007; 578(2): 413 - 424. [Abstract] [Full Text] [PDF]

				S. L. Zimmerman, J. Frisbie, D. L. Goldstein, J. West, K. Rivera, and C. M. Krane Excretion and conservation of glycerol, and expression of aquaporins and glyceroporins, during cold acclimation in Cope's gray tree frog Hyla chrysoscelis Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2007; 292(1): R544 - R555. [Abstract] [Full Text] [PDF]

				R. M. Vekaria, R. J. Unwin, and D. G. Shirley Intraluminal ATP Concentrations in Rat Renal Tubules J. Am. Soc. Nephrol., July 1, 2006; 17(7): 1841 - 1847. [Abstract] [Full Text] [PDF]

				R. Sun, R. L. Miller, A. C. Hemmert, P. Zhang, H. Shi, R. D. Nelson, and B. K. Kishore Chronic dDAVP infusion in rats decreases the expression of P2Y2 receptor in inner medulla and P2Y2 receptor-mediated PGE2 release by IMCD Am J Physiol Renal Physiol, October 1, 2005; 289(4): F768 - F776. [Abstract] [Full Text] [PDF]

				R. Sun, N. G. Carlson, A. C. Hemmert, and B. K. Kishore P2Y2 receptor-mediated release of prostaglandin E2 by IMCD is altered in hydrated and dehydrated rats: relevance to AVP-independent regulation of IMCD function Am J Physiol Renal Physiol, September 1, 2005; 289(3): F585 - F592. [Abstract] [Full Text] [PDF]