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In vivo stimulation of apical P2 receptors in collecting ducts: evidence for inhibition of sodium reabsorption

Centre for Nephrology and Department of Physiology, Royal Free and University College Medical School, London, United Kingdom

Submitted 30 April 2004 ; accepted in final form 23 January 2005

In vitro evidence suggests that intraluminal nucleotides, acting on apical P2 receptors, may influence amiloride-sensitive sodium reabsorption in collecting ducts. The present study has assessed this possibility directly in anesthetized rats, by determining the urinary recovery of ²²Na relative to that of [¹⁴C]inulin (Na/inulin recovery ratio) during in vivo microperfusion of late distal tubules with artificial tubular fluid containing various P2 agonists (all at 1 mM). In animals maintained on a control diet, in which amiloride-sensitive ²²Na reabsorption was modest, the poorly hydrolysable, broad-spectrum P2 agonist ATPS had no significant effect on the Na/inulin recovery ratio. In contrast, in rats maintained on a low-sodium diet, in which amiloride-sensitive ²²Na reabsorption was considerably enhanced, ATPS caused a significant increase in the Na/inulin recovery ratio (control: 14 ± 3%; ATPS: 28 ± 4%; n = 32 pairs; P < 0.001, paired t-test). No change in the Na/inulin recovery ratio was seen in time controls (13 ± 3 vs. 14 ± 4%; n = 15 pairs). In subsequent experiments in rats maintained on a low-sodium diet, we used more selective agonists in an attempt to identify the receptor subtype responsible for the effect of ATPS. The P2Y₁ agonist 2meSADP, the P2Y_2/4 agonists Ap₄A and Cp₄U, and the P2X agonist BzATP were all without significant effect on the Na/inulin recovery ratio. These findings constitute the first in vivo evidence for a functional role for apical P2 receptors in collecting ducts, but the identity of the receptor subtype(s) involved remains elusive.

purinoceptors; in vivo microperfusion; ATPS

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