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This Article Full Text Full Text (PDF) All Versions of this Article: 289/6/F1273    most recent 00103.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hader, C. E. Articles by Tremblay, J. Search for Related Content PubMed PubMed Citation Articles by Hader, C. E. Articles by Tremblay, J.

HCaRG increases renal cell migration by a TGF- autocrine loop mechanism

¹Laboratory of Cellular Biology of Hypertension and ⁴Laboratory of Molecular Medicine, Centre Hospitalier de l'Université de Montréal, ³Laboratory of Molecular Medicine, Hôpital Sainte-Justine-Université du Québec à Montréal, and ²Division of Experimental Medicine, McGill University, Montréal, Québec, Canada

Submitted 15 March 2005 ; accepted in final form 13 July 2005

We have shown previously that the hypertension-related, calcium-regulated gene (HCaRG) is involved in the control of renal cell proliferation and differentiation (Devlin AM, Solban N, Tremblay S, Gutkowska J, Schurch W, Orlov SN, Lewanczuk R, Hamet P, and Tremblay J. Am J Physiol Renal Physiol 284: F753–F762, 2003). To determine whether HCaRG plays a role in kidney repair after injury, we extended our studies on the cellular function of HCaRG by comparing cell migration of two kidney cell lines [HEK293 and Madin-Darby canine kidney (MDCK)-C7] stably transfected with the plasmid alone or with a plasmid containing HCaRG cDNA. HCaRG-expressing HEK293 cells, which undergo lower proliferation, migrated faster than control cells and presented greater adhesiveness to the extracellular matrix. Faster migration was also observed for the MDCK-C7 cells, after they were stably transfected with HCaRG cDNA. HCaRG overexpression induced major morphological changes in HEK293 cells, including the formation of lamellipodia. Expression microarrays of HCaRG-expressing HEK293 cells revealed the elevated expression of several genes known to be involved in cell migration and lamellipodia formation, including transforming growth factor- (TGF-), galectins, autotaxins and fibronectin. These cells exhibited augmented synthesis and release of activated TGF-. Conditioned medium from HCaRG-expressing cells stimulated the migration and induced significant morphological changes in control cells, in part, through activation of the TFG-/EGF receptor. Together, these data support a role for HCaRG in kidney repair after injury through its effect on renal cell migration and TGF- secretion.

hypertension-related, calcium-regulated gene; cell motility; kidney repair; lamellipodia; microarrays; kidney 293 cells; Madin-Darby canine kidney cells

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