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Am J Physiol Renal Physiol 289: F1281-F1290, 2005. First published August 2, 2005; doi:10.1152/ajprenal.00172.2005
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Genetic ablation of Rhbg in the mouse does not impair renal ammonium excretion

Régine Chambrey,1 Dominique Goossens,2 Soline Bourgeois,1 Nicolas Picard,1 May Bloch-Faure,3 Françoise Leviel,1,4 Valérie Geoffroy,5 Michele Cambillau,6 Yves Colin,2 Michel Paillard,1,4 Pascal Houillier,1,4 Jean Pierre Cartron,2 and Dominique Eladari1,7

1INSERM U652, IFR58, Institut des Cordeliers, Université René Descartes, 2INSERM U665, Institut National de la Transfusion Sanguine, 3UMR 7134 CNRS-Université Pierre et Marie Curie, 4Departement de Physiologie and 6Service de Biochimie, Hôpital Européen Georges Pompidou, AP-HP, 5INSERM U439, Hôpital Lariboisière, and 7Département de Physiologie, Hôpital Necker-Enfant Malades, AP-HP, Paris, France

Submitted 21 April 2005 ; accepted in final form 27 July 2005

NH4+ transport by the distal nephron and NH4+ detoxification by the liver are critical for achieving regulation of acid-base balance and to avoid hyperammonemic hepatic encephalopathy, respectively. Therefore, it has been proposed that rhesus type B glycoprotein (Rhbg), a member of the Mep/Amt/Rh NH3 channel superfamily, may be involved in some forms of distal tubular acidosis and congenital hyperammonemia. We have tested this hypothesis by inactivating the RHbg gene in the mouse by insertional mutagenesis. Histochemical studies analyses confirmed that RHbg knockout (KO) mice did not express Rhbg protein. Under basal conditions, the KO mice did not exhibit encephalopathy and survived well. They did not exhibit hallmarks of distal tubular acidosis because neither acid-base status, serum potassium concentration, nor bone mineral density was altered by RHbg disruption. They did not have hyperammonemia or disturbed hepatic NH3 metabolism. Moreover, the KO mice adapted to a chronic acid-loading challenge by increasing urinary NH4+ excretion as well as their wild-type controls. Finally, transepithelial NH3 diffusive permeability, or NH3 and NH4+ entry across the basolateral membrane of cortical collecting duct cells, measured by in vitro microperfusion of collecting duct from KO and wild-type mice, was identical with no apparent effect of the absence of Rhbg protein. We conclude that Rhbg is not a critical determinant of NH4+ excretion by the kidney and of NH4+ detoxification by the liver in vivo.

rhesus protein; acid-base; tubular acidosis; ammonia


Address for reprint requests and other correspondence: R. Chambrey, INSERM U652, IFR58, Institut des Cordeliers, 15 rue de l'Ecole de médecine, 75006 Paris, France (e-mail: chambrey{at}ccr.jussieu.fr) or D. Eladari, INSERM U652, IFR58, Institut des Cordeliers, 15 rue de l'Ecole de médecine, 75006 Paris, France (e-mail: eladari{at}ccr.jussieu.fr)




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