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This Article Full Text Full Text (PDF) All Versions of this Article: 290/4/F838    most recent 00374.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Related articles in AJP - Renal Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Weinman, E. J. Articles by Cunningham, R. Search for Related Content PubMed PubMed Citation Articles by Weinman, E. J. Articles by Cunningham, R.

Longitudinal study of urinary excretion of phosphate, calcium, and uric acid in mutant NHERF-1 null mice

¹Department of Medicine, ²Department of Physiology, University of Maryland School of Medicine, and ³Medical Service, Department of Veterans Affairs Medical Center, Baltimore; ⁴Harbor Hospital Center, Brooklyn, Maryland; and ⁵Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina

Submitted 15 September 2005 ; accepted in final form 23 October 2005

NHERF-1 binds numerous renal protein targets, including the proximal tubule transporters Na⁺/H⁺ exchanger 3 (NHE3) and Na⁺-phosphate cotransporter 2a (Npt2a). Young NHERF-1^–/– male mice display defective targeting of Npt2a to apical membranes in the renal proximal tubule and manifest hypophosphatemia and increased urinary excretion of phosphate. The present studies describe the changes in the urinary excretion of phosphate, calcium, uric acid, and sodium in male and female wild-type and NHERF-1 null mice over a time period from 12 to 54 wk of age. Young male and female NHERF-1^–/– mice demonstrated increased urinary excretion of phosphate and urine phosphate/creatinine ratios. There was an age-related decline in the phosphate/creatinine ratio in mutant mice such that there were no differences between wild-type and NHERF-1^–/– by 24 to 30 wk of age despite the continued presence of hypophosphatemia. Male and female NHERF-1 null mice also demonstrate increased urine calcium/creatinine and uric acid/creatinine ratios compared with wild-type controls. These studies indicate defects in the renal tubule transport of phosphate, calcium, and uric acid in NHERF-1^–/– male and female mice that could account for the increased deposition of calcium in the papilla of null mice.

bone density and mineralization; hypophosphatemia; electrolyte excretion

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Corrigenda

AJP - Renal 2006 291: F254-F255. [Full Text]  

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