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This Article Full Text Full Text (PDF) All Versions of this Article: 290/5/F1143    most recent 00333.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Ruan, H. Z. Articles by Burnstock, G. Search for Related Content PubMed PubMed Citation Articles by Ruan, H. Z. Articles by Burnstock, G.

Expression of P2X and P2Y receptors in the intramural parasympathetic ganglia of the cat urinary bladder

¹Autonomic Neuroscience Centre, Royal Free and University College Medical School, London, United Kingdom; ²Department of Neurobiology, Third Military Medical University, Chongqing; Departments of ³Medicine and ⁴Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; ⁶Department of Clinical Veterinary Sciences, Ohio State University, Columbus, Ohio; and ⁵Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai, People's Republic of China

Submitted 17 August 2005 ; accepted in final form 29 November 2005

The distribution and function of P2X and P2Y receptor subtypes were investigated on intact or cultured intramural ganglia of the cat urinary bladder by immunocytochemistry and calcium-imaging techniques, respectively. Neurons were labeled by all seven P2X receptor subtype antibodies and antibodies for P2Y₂, P2Y₄, P2Y₆, and P2Y₁₂ receptor subtypes with a staining intensity of immunoreactivity in the following order: P2X₃=P2Y₂=P2Y₄=P2Y₆=P2Y₁₂>P2X₁=P2X₂=P2X₄>P2X₅=P2X₆=P2X₇. P2Y₁ receptor antibodies labeled glial cells, but not neurons. P2X₃ and P2Y₄ polyclonal antibodies labeled 95 and 40% of neurons, respectively. Double staining showed that 100, 48.8, and 97.4% of P2X₃ receptor-positive neurons coexpressed choline acetyl transferase (ChAT), nitric oxide synthase (NOS), and neurofilament 200 (NF200), respectively, whereas 100, 59.2, and 97.6% of P2Y₄ receptor-positive neurons coexpressed ChAT, NOS, and NF200, respectively. Application of ATP, ,-methylene ATP, and uridine triphosphate elevated intracellular Ca²⁺ concentration in a subpopulation of dissociated cultured cat intramural ganglia neurons, demonstrating the presence of functional P2Y₄ and P2X₃ receptors. This study indicates that P2X and P2Y receptor subtypes are expressed by cholinergic parasympathetic neurons innervating the urinary bladder. The neurons were also stained for NF200, usually regarded as a marker for large sensory neurons. These novel histochemical properties of cholinergic neurons in the cat bladder suggest that the parasympathetic pathways to the cat bladder may be modulated by complex purinergic synaptic mechanisms.

ATP; intramural ganglia

This article has been cited by other articles:

				G. Burnstock Physiology and Pathophysiology of Purinergic Neurotransmission Physiol Rev, April 1, 2007; 87(2): 659 - 797. [Abstract] [Full Text] [PDF]