HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/2/F503    most recent 00482.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Sandberg, M. B. Articles by McDonough, A. A. Search for Related Content PubMed PubMed Citation Articles by Sandberg, M. B. Articles by McDonough, A. A.

REPORT

Redistribution of distal tubule Na⁺-Cl^– cotransporter (NCC) in response to a high-salt diet

¹Department of Physiology and Biophysics, University of Southern California Keck School of Medicine, Los Angeles, California; and ²The Water and Salt Research Center, Department of Cell Biology, Institute of Anatomy, University of Aarhus, Aarhus C, Denmark

Submitted 5 December 2005 ; accepted in final form 7 March 2006

ABSTRACT

The distal convoluted tubule (DCT) apical Na⁺-Cl^– cotransporter (NCC) is responsible for the reabsorption of 5–10% of filtered NaCl and is the target for thiazide diuretics. NCC abundance is increased during dietary NaCl restriction and by aldosterone and decreased during a high-salt (HS) diet and mineralocorticoid blockade. This study tested the hypothesis that subcellular distribution of NCC is also regulated in response to changes in dietary salt. Six-week-old Sprague-Dawley rats were fed a normal-salt diet (NS; 0.4% NaCl) for 3 wk, then switched to a HS diet (4% NaCl) for 3 wk or a low-salt diet (LS; 0.07% NaCl) for 1 wk. Under anesthesia, kidneys were excised, renal cortex was dissected, and NCC was analyzed with specific antibodies after either 1) density gradient centrifugation followed by immunoblotting or 2) fixation followed by immunoelectron microscopy. The HS diet decreased NCC abundance to 0.50 ± 0.10 of levels in LS diet (1.00 ± 0.23). The HS diet also caused a redistribution of NCC from low to higher density membranes. Immunoelectron microscopy revealed that NCC resides predominantly in the apical membrane in rats fed the LS diet and increases in subapical vesicles in rats fed the HS diet. In conclusion, a HS diet provokes a rapid and persistent redistribution of NCC from apical to subapical membranes, a mechanism that would facilitate a homeostatic decrease in NaCl reabsorption in the DCT to compensate for increased dietary salt.

sodium transport; distal convoluted tubule; dietary salt; thiazide receptor

This article has been cited by other articles:

				D. H. Lee, A. D. M. Riquier, L. E. Yang, P. K. K. Leong, A. B. Maunsbach, and A. A. McDonough Acute hypertension provokes acute trafficking of distal tubule Na-Cl cotransporter (NCC) to subapical cytoplasmic vesicles Am J Physiol Renal Physiol, April 1, 2009; 296(4): F810 - F818. [Abstract] [Full Text] [PDF]

				E. Riveira-Munoz, O. Devuyst, H. Belge, N. Jeck, L. Strompf, R. Vargas-Poussou, X. Jeunemaitre, A. Blanchard, N. V. Knoers, M. Konrad, et al. Evaluating PVALB as a candidate gene for SLC12A3-negative cases of Gitelman's syndrome Nephrol. Dial. Transplant., October 1, 2008; 23(10): 3120 - 3125. [Abstract] [Full Text] [PDF]

				L. E. Yang, M. B. Sandberg, A. D. Can, K. Pihakaski-Maunsbach, and A. A. McDonough Effects of dietary salt on renal Na+ transporter subcellular distribution, abundance, and phosphorylation status Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1003 - F1016. [Abstract] [Full Text] [PDF]

				L. E. Yang, P. K. K. Leong, and A. A. McDonough Reducing blood pressure in SHR with enalapril provokes redistribution of NHE3, NaPi2, and NCC and decreases NaPi2 and ACE abundance Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1197 - F1208. [Abstract] [Full Text] [PDF]

				M. B. Sandberg, A. D. M. Riquier, K. Pihakaski-Maunsbach, A. A. McDonough, and A. B. Maunsbach ANG II provokes acute trafficking of distal tubule Na+-Cl cotransporter to apical membrane Am J Physiol Renal Physiol, September 1, 2007; 293(3): F662 - F669. [Abstract] [Full Text] [PDF]

				J. W. Verlander The thiazide-sensitive NaCl cotransporter: a new target for acute regulation of salt and water transport by angiotensin II Am J Physiol Renal Physiol, September 1, 2007; 293(3): F660 - F661. [Full Text] [PDF]

				J. Nielsen, T.-H. Kwon, J. Frokiaer, M. A. Knepper, and S. Nielsen Maintained ENaC trafficking in aldosterone-infused rats during mineralocorticoid and glucocorticoid receptor blockade Am J Physiol Renal Physiol, January 1, 2007; 292(1): F382 - F394. [Abstract] [Full Text] [PDF]