|
|
||||||||
and
ENaC
Department of Physiology and Biophysics and the Center for Excellence in Cardiovascular Renal Research, University of Mississippi Medical Center, Jackson, Mississippi
Submitted 23 May 2006 ; accepted in final form 12 July 2006
Mechanosensitive ion channels are thought to initiate pressure-induced vasoconstriction, however, the molecular identity of these channels is unknown. Recent work from our laboratory suggests that members of the Degenerin/Epithelial Na+ Channel (DEG/ENaC) family may be components of the mechanosensitive ion channel complex in vascular smooth muscle (VSM); however, the specific DEG/ENaC proteins mediating myogenic constriction are unknown. The goal of this study is to determine if specific knockdown of
or
ENaC, using dominant-negative (DN) or small-interference RNA (siRNA) molecules, inhibits pressure-induced vasoconstriction in mouse renal interlobar arteries. To address this goal, isolated arteries were transiently transfected with
or
ENaC DN-cDNA or siRNA molecules. After 24 h, vessels were either 1) cannulated and pressurized for pressure-diameter response curves or 2) dissociated and immunolabeled to determine VSM cell endogenous ENaC protein expression. We found that transfection of
ENaC DN-cDNA or siRNA suppresses
-, but not
ENaC protein expression. Similarly,
ENaC DN-cDNA or siRNA suppresses
-, but not
ENaC protein expression. In addition, transfection of
- or
ENaC DN-cDNA or siRNA molecules inhibits pressure-induced vasoconstriction, but does not block agonist-induced vasoconstriction. Our results provide the first direct evidence that
and
ENaC proteins are essential in mediating myogenic vasoconstriction in mouse renal interlobar arteries.
mechanotransduction; transfected isolated renal vessel; stretch-activated cation channel; siRNA; dominant negative
This article has been cited by other articles:
![]() |
J. L. McCord, S. G. Hayes, and M. P. Kaufman Acid-sensing ion and epithelial sodium channels do not contribute to the mechanoreceptor component of the exercise pressor reflex Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H1017 - H1024. [Abstract] [Full Text] [PDF] |
||||
![]() |
X. Wang, K. Takeya, P. I. Aaronson, K. Loutzenhiser, and R. Loutzenhiser Effects of amiloride, benzamil, and alterations in extracellular Na+ on the rat afferent arteriole and its myogenic response Am J Physiol Renal Physiol, July 1, 2008; 295(1): F272 - F282. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. C. Grifoni, S. E. McKey, and H. A. Drummond Hsc70 regulates cell surface ASIC2 expression and vascular smooth muscle cell migration Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2022 - H2030. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. A. Drummond, N. L. Jernigan, and S. C. Grifoni Sensing Tension: Epithelial Sodium Channel/Acid-Sensing Ion Channel Proteins in Cardiovascular Homeostasis Hypertension, May 1, 2008; 51(5): 1265 - 1271. [Full Text] [PDF] |
||||
![]() |
K. P. Gannon, L. G. VanLandingham, N. L. Jernigan, S. C. Grifoni, G. Hamilton, and H. A. Drummond Impaired pressure-induced constriction in mouse middle cerebral arteries of ASIC2 knockout mice Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1793 - H1803. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. A. Drummond, S. C. Grifoni, and N. L. Jernigan A New Trick for an Old Dogma: ENaC Proteins as Mechanotransducers in Vascular Smooth Muscle Physiology, February 1, 2008; 23(1): 23 - 31. [Abstract] [Full Text] [PDF] |
||||
![]() |
R. Schubert, D. Lidington, and S.-S. Bolz The emerging role of Ca2+ sensitivity regulation in promoting myogenic vasoconstriction Cardiovasc Res, January 1, 2008; 77(1): 8 - 18. [Abstract] [Full Text] [PDF] |
||||
![]() |
N. L. Jernigan, B. LaMarca, J. Speed, L. Galmiche, J. P. Granger, and H. A. Drummond Dietary salt enhances benzamil-sensitive component of myogenic constriction in mesenteric arteries Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H409 - H420. [Abstract] [Full Text] [PDF] |
||||
![]() |
H. A. Coleman and H. C. Parkington Endothelin enhances activity of mechanosensitive channels: A mechanism for ET augmentation of the myogenic response Cardiovasc Res, November 1, 2007; 76(2): 197 - 198. [Full Text] [PDF] |
||||
![]() |
C. J. Busst, K. J. Scurrah, J. A. Ellis, and S. B. Harrap Selective Genotyping Reveals Association Between the Epithelial Sodium Channel {gamma}-Subunit and Systolic Blood Pressure Hypertension, October 1, 2007; 50(4): 672 - 678. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |