HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 292/1/F351    most recent 00049.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Carmosino, M. Articles by Breyer, M. D. Search for Related Content PubMed PubMed Citation Articles by Carmosino, M. Articles by Breyer, M. D.

Axial heterogeneity of vasopressin-receptor subtypes along the human and mouse collecting duct

¹Division of Nephrology, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee; ²Department of Physiology, University of Arizona, Tucson, Arizona; and ³Department of Pathology, Vanderbilt University Medical Center, Nashville, Tennessee

Submitted 13 February 2006 ; accepted in final form 19 June 2006

Vasopressin and vasopressin antagonists are finding expanded use in mouse models of disease and in clinical medicine. To provide further insight into the physiological role of V1a and V2 vasopressin receptors in the human and mouse kidney, intrarenal localization of the receptors mRNA was determined by in situ hybridization. V2-receptor mRNA was predominantly expressed in the medulla, whereas mRNA for V1a receptors predominated in the cortex. The segmental localization of vasopressin-receptor mRNAs was determined using simultaneous in situ hybridization and immunohistochemistry for segment-specific markers, including aquaporin-2, Dolichos biflorus agglutinin, epithelial Na channels, Tamm Horsfall glycoprotein, and thiazide-sensitive Na⁺-Cl^– cotransporter. Notably, V1a receptor expression was exclusively expressed in V-ATPase/anion exchanger-1-labeled alpha-intercalated cells of the medullary collecting duct in both mouse and human kidney. In cortical collecting ducts, V1a mRNA was more widespread and detected in both principal and intercalated cells. V2-receptor mRNA is diffusely expressed along the collecting ducts in both mouse and human kidney, with higher expression levels in the medulla. These results demonstrate heterogenous axial expression of both V1a and V2 vasopressin receptors along the human and mouse collecting duct. The restricted expression of V1a-receptor mRNA in intercalated cells suggests a role for this receptor in acid-base balance. These findings further suggest distinct regulation of renal transport function by AVP through V1a and V2 receptors in the cortex vs. the medulla.

in situ hybridization; vasopressin receptors; intercalated cell

This article has been cited by other articles:

				Y. Izumi, Y. Nakayama, H. Memetimin, T. Inoue, Y. Kohda, H. Nonoguchi, and K. Tomita Regulation of V2R transcription by hypertonicity and V1aR-V2R signal interaction Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1170 - F1176. [Abstract] [Full Text] [PDF]

				T. Aoyagi, Y. Izumi, M. Hiroyama, T. Matsuzaki, Y. Yasuoka, A. Sanbe, H. Miyazaki, Y. Fujiwara, Y. Nakayama, Y. Kohda, et al. Vasopressin regulates the renin-angiotensin-aldosterone system via V1a receptors in macula densa cells Am J Physiol Renal Physiol, July 1, 2008; 295(1): F100 - F107. [Abstract] [Full Text] [PDF]

				K. Mutig, A. Paliege, T. Kahl, T. Jons, W. Muller-Esterl, and S. Bachmann Vasopressin V2 receptor expression along rat, mouse, and human renal epithelia with focus on TAL Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1166 - F1177. [Abstract] [Full Text] [PDF]

				R. A. Fenton, L. Brond, S. Nielsen, and J. Praetorius Cellular and subcellular distribution of the type-2 vasopressin receptor in the kidney Am J Physiol Renal Physiol, September 1, 2007; 293(3): F748 - F760. [Abstract] [Full Text] [PDF]