HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) A corrigendum has been published All Versions of this Article: 292/1/F361    most recent 00207.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Ljubojevic, M. Articles by Sabolic, I. Search for Related Content PubMed PubMed Citation Articles by Ljubojevic, M. Articles by Sabolic, I.

Renal expression of organic anion transporter OAT2 in rats and mice is regulated by sex hormones

Marija Ljubojevi,¹ Daniela Balen,¹ Davorka Breljak,¹ Marija Kuan,² Naohiko Anzai,³ Andrew Bahn,⁴ Gerhard Burckhardt,⁴ and Ivan Saboli¹

¹Molecular Toxicology, Institute for Medical Research and Occupational Health, Zagreb, Croatia; ²Physiology, School of Medicine, University of Zagreb, Zagreb, Croatia; ³Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo, Japan; and ⁴Vegetative Physiology and Pathophysiology, University of Göttingen, Göttingen, Germany

Submitted 8 June 2006 ; accepted in final form 27 July 2006

The renal reabsorption and/or excretion of various organic anions is mediated by specific organic anion transporters (OATs). OAT2 (Slc22a7) has been identified in rat kidney, where its mRNA expression exhibits gender differences [females (F) > males (M)]. The exact localization of OAT2 protein in the mammalian kidney has not been reported. Here we studied the expression of OAT2 mRNA by RT-PCR and its protein by Western blotting (WB) and immunocytochemistry (IC) in kidneys of adult intact and gonadectomized M and F, sex hormone-treated castrated M, and prepubertal M and F rats, and the protein in adult M and F mice. In adult rats, the expression of OAT2 mRNA was predominant in the outer stripe (OS) tissue, exhibiting 1) gender dependency (F > M), 2) upregulation by castration and downregulation by ovariectomy, and 3) strong downregulation by testosterone and weak upregulation by estradiol and progesterone treatment. A polyclonal antibody against rat OAT2 on WB of isolated renal membranes labeled a 66-kDa protein band that was stronger in F. By IC, the antibody exclusively stained brush border (BB) of the proximal tubule S3 segment (S3) in the OS and medullary rays (F > M). In variously treated rats, the pattern of 66-kDa band density in the OS membranes and the staining intensity of BB in S3 matched the mRNA expression. The expression of OAT2 protein in prepubertal rats was low and gender independent. In mice, the expression pattern largely resembled that in rats. Therefore, OAT2 in rat (and mouse) kidney is localized to the BB of S3, exhibiting gender differences (F > M) that appear in puberty and are caused by strong androgen inhibition and weak estrogen and progesterone stimulation.

androgens; estrogens; gender differences; kidney; membrane transporters; progesterone; organic anion; transporter-2

This article has been cited by other articles:

				D. Balen, M. Ljubojevic, D. Breljak, H. Brzica, V. Zlender, H. Koepsell, and I. Sabolic Revised immunolocalization of the Na+-D-glucose cotransporter SGLT1 in rat organs with an improved antibody Am J Physiol Cell Physiol, August 1, 2008; 295(2): C475 - C489. [Abstract] [Full Text] [PDF]