|
|
||||||||
National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland
Submitted 11 August 2006 ; accepted in final form 1 September 2006
In the current experiments, we determined the response of plasma renin concentration (PRC) to acute intraperitoneal administration of furosemide (40 mg/kg), hydralazine (2 mg/kg), isoproterenol (10 mg/kg), candesartan (50 µg), or quinaprilate (50 µg) in conscious wild-type (WT) and cyclooxygenase (COX)-2/ mice on three different genetic backgrounds (mixed, C57BL/6, 129J). PRC was measured in plasma obtained by tail vein puncture. Basal PRC was significantly lower in COX-2/ than WT mice independent of genetic background (51, 10, and 17% of WT in mixed, 129J, and C57BL/6). All five acute interventions caused significant increases of PRC in both COX-2+/+ and / mice, but the response was consistently less in COX-2-deficient mice (e.g.,
PRC in ng ANG I·ml1·h1 caused by furosemide, isoproterenol, hydralazine, quinaprilate, or candesartan 4,699 ± 544, 3,534 ± 957, 2,522 ± 369, 9,453 ± 1,705, 66,455 ± 21,938 in 129J WT, and 201 ± 78, 869 ± 275, 140 ± 71, 902 ± 304, 2,660 ± 954 in 129J COX-2/). A low-NaCl diet and enalapril for 1 wk caused a 14-fold elevation of PRC in COX-2/ mice and was associated with a greatly increased PRC response to acute furosemide (
PRC 201 ± 78 before and 15,984 ± 2,397 after low Na/enalapril). As measured by radiotelemetry, blood pressure and heart rate responses to furosemide, hydralazine, isoproterenol, candesartan, or quinaprilate were not different between COX-2 genotypes. In conclusion, chronic absence of COX-2 reduces renin expression, release, and PRC and is associated with a reduced ability to alter PRC during acute stimulation regardless of the nature of the stimulus. COX-2 activity does not appear to be a mandatory and specific requirement for furosemide-stimulated renin secretion.
furosemide; hydralazine; isoproterenol; quinaprilate; candesartan; genetic background; cyclooxygenase-2
This article has been cited by other articles:
![]() |
B. Neubauer, K. Machura, M. Chen, L. S. Weinstein, M. Oppermann, M. L. Sequeira-Lopez, R. A. Gomez, J. Schnermann, H. Castrop, A. Kurtz, et al. Development of vascular renin expression in the kidney critically depends on the cyclic AMP pathway Am J Physiol Renal Physiol, May 1, 2009; 296(5): F1006 - F1012. [Abstract] [Full Text] [PDF] |
||||
![]() |
M.-Z. Zhang, B. Yao, X. Fang, S. Wang, J. P. Smith, and R. C. Harris Intrarenal Dopaminergic System Regulates Renin Expression Hypertension, March 1, 2009; 53(3): 564 - 570. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. M. Kim, F. Theilig, Y. Qin, T. Cai, D. Mizel, R. Faulhaber-Walter, H. Hirai, S. Bachmann, J. P. Briggs, A. L. Notkins, et al. Dense-core vesicle proteins IA-2 and IA-2{beta} affect renin synthesis and secretion through the {beta}-adrenergic pathway Am J Physiol Renal Physiol, February 1, 2009; 296(2): F382 - F389. [Abstract] [Full Text] [PDF] |
||||
![]() |
Z. A. Radi Pathophysiology of Cyclooxygenase Inhibition in Animal Models Toxicol Pathol, January 1, 2009; 37(1): 34 - 46. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. M. Bivol, M. Hultstrom, O. A. Gudbrandsen, R. K. Berge, and B. M. Iversen Tetradecylthioacetic acid downregulates cyclooxygenase 2 in the renal cortex of two-kidney, one-clip hypertensive rats Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2008; 295(6): R1866 - R1873. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Kantorowicz, N. K. Valego, LiJun Tang, J. P. Figueroa, M. C. Chappell, L. C. Carey, and J. C. Rose Plasma and Renal Renin Concentrations in Adult Sheep After Prenatal Betamethasone Exposure Reproductive Sciences, October 1, 2008; 15(8): 831 - 838. [Abstract] [PDF] |
||||
![]() |
R. Faulhaber-Walter, L. Chen, M. Oppermann, S. M. Kim, Y. Huang, N. Hiramatsu, D. Mizel, H. Kajiyama, P. Zerfas, J. P. Briggs, et al. Lack of A1 Adenosine Receptors Augments Diabetic Hyperfiltration and Glomerular Injury J. Am. Soc. Nephrol., April 1, 2008; 19(4): 722 - 730. [Abstract] [Full Text] [PDF] |
||||
![]() |
C. Wagner, C. de Wit, M. Gerl, A. Kurtz, and K. Hocherl Increased expression of cyclooxygenase 2 contributes to aberrant renin production in connexin 40-deficient kidneys Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R1781 - R1786. [Abstract] [Full Text] [PDF] |
||||
![]() |
S. M. Kim, L. Chen, R. Faulhaber-Walter, M. Oppermann, Y. Huang, D. Mizel, J. P. Briggs, and J. Schnermann Regulation of Renin Secretion and Expression in Mice Deficient in {beta}1- and {beta}2-Adrenergic Receptors Hypertension, July 1, 2007; 50(1): 103 - 109. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |