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This Article Full Text Full Text (PDF) All Versions of this Article: 292/1/F486    most recent 00188.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (2) Citing Articles via Google Scholar Google Scholar Articles by Náray-Fejes-Tóth, A. Articles by Fejes-Tóth, G. Search for Related Content PubMed PubMed Citation Articles by Náray-Fejes-Tóth, A. Articles by Fejes-Tóth, G.

INNOVATIVE METHODOLOGY

Novel mouse strain with Cre recombinase in 11-hydroxysteroid dehydrogenase-2-expressing cells

Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire

Submitted 28 May 2006 ; accepted in final form 30 July 2006

Here we describe the generation and characterization of a mouse strain that expresses an improved Cre (iCre) recombinase (48) under the control of the endogenous 11-hydroxysteroid dehydrogenase type 2 (11HSD2) promoter. Progeny of 11HSD2/iCre and ROSA26 reporter mice were used to determine the pattern of iCre expression by measuring the activity of the LacZ gene product -galactosidase in a panel of tissues. On Cre recombinase activity, intense -galactosidase activity (X-gal staining) was observed in the classic mineralocorticoid target segments of the kidney, as well as in the colon, and both female and male reproductive organs. Weaker iCre expression was detected in the lung and heart. In the brain, strong iCre activity was present in cardiovascular centers that are known to express 11HSD2 and mineralocorticoid receptors [nucleus tractus solitarius (NTS) and amygdala] as well as in the granular layer of the cerebellum. iCre expression was weaker in neonatal kidney and colon than in the adult but was present in the hair follicles and cartilage. These results indicate that in the 11HSD2/iCre strain iCre expression faithfully represents the expression pattern of endogenous 11HSD2. Thus this mouse model represents the first Cre deleter strain that can be used to eliminate desired genes in every mineralocorticoid target tissue. This mouse model should serve as a useful resource for investigators who want to study the function of genes involved in aldosterone action and genes in other pathways that are selectively expressed in these cells.

aldosterone; loxP; collecting duct; kidney

This article has been cited by other articles:

				J. C. Geerling and A. D. Loewy 11beta-Hydroxysteroid dehydrogenase 2 vs. transgene: discrepant loci of expression in the adult brain Am J Physiol Renal Physiol, July 1, 2007; 293(1): F440 - F441. [Full Text] [PDF]

				A. Naray-Fejes-Toth and G. Fejes-Toth Reply to Geerling and Loewy Am J Physiol Renal Physiol, July 1, 2007; 293(1): F442 - F443. [Full Text] [PDF]