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Renal compensation to chronic hypoxic hypercapnia: downregulation of pendrin and adaptation of the proximal tubule

¹The Water and Salt Research Center, ²Institute of Anatomy, and ³Zoophysiology, Department of Biological Sciences, University of Aarhus; and ⁴Institute of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark

Submitted 16 June 2006 ; accepted in final form 6 December 2006

The molecular basis for the renal compensation to respiratory acidosis and specifically the role of pendrin in this condition are unclear. Therefore, we studied the adaptation of the proximal tubule and the collecting duct to respiratory acidosis. Male Wistar-Hannover rats were exposed to either hypercapnia and hypoxia [8% CO₂ and 13% O₂ (hypercapnic, n = 6) or normal air (controls, n = 6)] in an environmental chamber for 10 days and were killed under the same atmosphere. In hypercapnic rats, arterial pH was lower than controls (7.31 ± 0.01 vs. 7.39 ± 0.01, P = 0.03), blood HCO₃^– concentration was increased (42 ± 0.9 vs. 32 ± 0.24 mM, P < 0.001), arterial PCO₂ was increased (10.76 ± 0.4 vs. 7.20 ± 0.4 kPa, P < 0.001), and plasma chloride concentration was decreased (92.2 ± 0.7 vs. 97.2 ± 0.5 mM, P < 0.001). Plasma aldosterone levels were unchanged. In the proximal tubule, immunoblotting showed an increased expression of sodium/bicarbonate exchanger protein (188 ± 22 vs. 100 ± 11%, P = 0.005), confirmed by immunohistochemistry. Total Na/H exchanger protein expression in the cortex was unchanged by immunoblotting (119 ± 10 vs. 100 ± 11%, P = 0.27) and immunohistochemistry. In the cortex, the abundance of pendrin was decreased (51 ± 9 vs. 100 ± 7%, P = 0.003) by immunoblotting. Immunohistochemistry revealed that this decrease was clear in both cortical collecting ducts (CCDs) and connecting tubules (CNTs). This demonstrates that pendrin expression can be regulated in acidotic animals with no changes in aldosterone levels and no external chloride load. This reduction of pendrin expression may help in redirecting the CNT and CCD toward chloride excretion and bicarbonate reabsorption, contributing to the increased plasma bicarbonate and decreased plasma chloride of chronic respiratory acidosis.

cortical collecting duct; chloride regulation; intercalated cells; bicarbonate

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