AJP - Renal Watch the video to learn how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol 295: F1254-F1258, 2008. First published August 13, 2008; doi:10.1152/ajprenal.00134.2008
0363-6127/08 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Tables
Right arrow All Versions of this Article:
295/4/F1254    most recent
00134.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Merchant, M. L.
Right arrow Articles by Lederer, E. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Merchant, M. L.
Right arrow Articles by Lederer, E. D.

REPORT

Proteomic analysis of renal calculi indicates an important role for inflammatory processes in calcium stone formation

Michael L. Merchant,1 Timothy D. Cummins,2 Daniel W. Wilkey,1 Sarah A. Salyer,1 David W. Powell,1,2 Jon B. Klein,1,2,3,4 and Eleanor D. Lederer1,4

1Department of Medicine, 2Department of Biochemistry and Molecular Biology, and 3James Graham Brown Cancer Center, University of Louisville and 4Department of Veterans Affairs Medical Center, Louisville, Kentucky

Submitted 13 March 2008 ; accepted in final form 11 August 2008

ABSTRACT

Even though renal stones/calculi occur in ~10% of individuals, they are an enormous economic burden to the entire US health system. While the relative metabolic composition of renal calculi is generally known, there is no clear understanding of the genetics of renal stone formation, nor are there clear prognostic indicators of renal stone formation. The application of proteomics to the analysis of renal calculi axiomatically holds that insight into renal stone pathobiology can be gained by a more comprehensive understanding of renal calculus protein composition. We analyzed isolated renal stone matrix proteins with mass spectrometric and immunohistochemical methods identifying 158 proteins with high confidence, including 28 common proteins. The abundant proteins included those identified previously in stones and proteins identified here for the first time, such as myeloid lineage-specific, integral membrane and lipid regulatory proteins. Pathway analyses of all proteins identified suggested that a significant fraction of the most abundant matrix proteins participate in inflammatory processes. These proteomic results support the hypothesis that stone formation induces a cellular inflammatory response and the protein components of this response contribute to the abundant stone matrix proteome.

kidney stones; osteopontin; inflammation; lipoprotein


Address for reprint requests and other correspondence: M. L. Merchant and E. D. Lederer, Rm. 102S, Donald Baxter Research Bldg., 570 S. Preston St., Louisville, KY 40202 (e-mail: mlmerc02{at}gwise.louisville.edu, edlede01{at}gwise.louisville.edu)




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
K. S. Sfanos, B. A. Wilson, A. M. De Marzo, and W. B. Isaacs
Acute inflammatory proteins constitute the organic matrix of prostatic corpora amylacea and calculi in men with prostate cancer
PNAS, March 3, 2009; 106(9): 3443 - 3448.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2008 by the American Physiological Society.