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This Article Full Text Full Text (PDF) All Versions of this Article: 295/5/F1324    most recent 00126.2008v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Peng, H.-Y. Articles by Lin, T.-B. Search for Related Content PubMed PubMed Citation Articles by Peng, H.-Y. Articles by Lin, T.-B.

TRPV₁ mediates the uterine capsaicin-induced NMDA NR2B-dependent cross-organ reflex sensitization in anesthetized rats

¹Department of Physiology, ³Department of Anatomy, College of Medicine and ⁶Department of Obstetrics and Gynecology, Chung-Shan Medical University Hospital, Chung-Shan Medical University, Taichung; ²Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung; ⁴Department of Physical Therapy and Graduate Institute of Rehabilitation Science, China Medical University, Taichung; ⁵Department of Obstetrics and Gynecology, Chang-Gung Memorial Hospital, Taoyuan; ⁷Medical Department, Saint Paul's Hospital, Taoyuan; and ⁸Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan

Submitted 11 March 2008 ; accepted in final form 9 July 2008

Spinal cord-mediated cross-organ sensitization between the uterus and the lower urinary tract may underlie the high concurrence of obstetrical/gynecological inflammation and chronic pelvic pain syndrome characterized by urogenital pain. However, the neural pathway and the neurotransmitters involved are still unknown. We tested the hypothesis that the excitation of capsaicin-sensitive primary afferent fibers arising from the uterus through the stimulation of transient receptor potential vanilloid 1 (TRPV₁) induces cross-organ sensitization on the pelvic-urethra reflex activity. Capsaicin (1–1,000 µM, 0.05 ml) was instilled into the uterus to induce cross-organ reflex sensitization. Activation of capsaicin-sensitive primary afferent fibers by capsaicin instillation into the uterine horn sensitized the pelvic-urethra reflex activity that was reversed by an intrauterine pretreatment with capsaizepine, a TRPV₁-selective antagonist. Intrathecal injection of AP5, a glutamatergic N-methyl-D-aspartate (NMDA) antagonist, and Co-101244, an NMDA NR2B-selective antagonist, both abolished the cross-organ reflex sensitization caused by capsaicin instillation. These results demonstrated that TRPV₁ plays a crucial role in contributing to the capsaicin-sensitive primary afferent fibers mediating the glutamatergic NMDA-dependent cross-organ sensitization between the uterus and the lower urinary tract when there is a tissue injury.

spinal cord; C-fiber; pain; desensitization

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