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This Article Full Text Full Text (PDF) All Versions of this Article: 296/2/F249    most recent 00612.2007v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mérillat, A.-M. Articles by Hummler, E. Search for Related Content PubMed PubMed Citation Articles by Mérillat, A.-M. Articles by Hummler, E.

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Aldosterone and Epithelial Na⁺ Channel

Conditional gene targeting of the ENaC subunit genes Scnn1b and Scnn1g

¹Département de Pharmacologie et de Toxicologie, ²Transgenic Animal Facility, and ³Service de Nephrologie du Centre Hospitalier Universitaire Vaudois, University of Lausanne, Lausanne; and ⁴ISREC (Swiss Institute for Experimental Cancer Research), School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Epalinges, Switzerland

Submitted 24 December 2007 ; accepted in final form 22 November 2008

Epithelial sodium channels (ENaC) are members of the degenerin/ENaC superfamily of non-voltage-gated, highly amiloride-sensitive cation channels that are composed of three subunits (-, β-, and -ENaC). Since complete gene inactivation of the β- and -ENaC subunit genes (Scnn1b and Scnn1g) leads to early postnatal death, we generated conditional alleles and obtained mice harboring floxed and null alleles for both gene loci. Using quantitative RT-PCR analysis, we showed that the introduction of the loxP sites did not interfere with the mRNA transcript expression level of the Scnn1b and Scnn1g gene locus, respectively. Upon a regular and salt-deficient diet, both β- and -ENaC floxed mice showed no difference in their mRNA transcript expression levels, plasma electrolytes, and aldosterone concentrations as well as weight changes compared with control animals. These mice can now be utilized to dissect the role of ENaC function in classical and nonclassic target organs/tissues.

transgenic; conditional knockout; epithelial sodium channel; amiloride-sensitive sodium channel; mouse model; β-ENaC; -ENaC