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Am J Physiol Renal Physiol 296: F642-F648, 2009. First published December 3, 2008; doi:10.1152/ajprenal.90660.2008
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Vasopressin regulation of the renal UT-A3 urea transporter

G. S. Stewart,3 A. Thistlethwaite,1 H. Lees,1 G. J. Cooper,2 and Craig Smith1

1Faculty of Life Sciences, Core Technology Facility, University of Manchester, Manchester; 2Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, United Kingdom; and 3School of Biology and Environmental Science, University College Dublin, Dublin, Ireland

Submitted 6 November 2008 ; accepted in final form 25 November 2008

Facilitative urea transporters in the mammalian kidney play a vital role in the urinary concentrating mechanism. The urea transporters located in the renal inner medullary collecting duct, namely UT-A1 and UT-A3, are acutely regulated by the antidiuretic hormone vasopressin. In this study, we investigated the vasopressin regulation of the basolateral urea transporter UT-A3 using an MDCK-mUT-A3 cell line. Within 10 min, vasopressin stimulates urea flux through UT-A3 transporters already present at the plasma membrane, via a PKA-dependent process. Within 1 h, vasopressin significantly increases UT-A3 localization at the basolateral membrane, causing a further increase in urea transport. While the basic trafficking of UT-A3 to basolateral membranes involves both protein kinase C and calmodulin, its regulation by vasopressin specifically occurs through a casein kinase II-dependent pathway. In conclusion, this study details the effects of vasopressin on UT-A3 urea transporter function and hence its role in regulating urea permeability within the renal inner medullary collecting duct.

arginine vasopressin; urea transport; membrane localization; casein kinase II


Address for reprint requests and other correspondence: C. Smith, Faculty of Life Sciences, 2nd Floor, Core Technology Facility, Univ. of Manchester, Grafton St., Manchester, M13 9NT, UK (e-mail: craig.smith{at}manchester.ac.uk)




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 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. Tickle, A. Thistlethwaite, C. P. Smith, and G. S. Stewart
Novel bUT-B2 urea transporter isoform is constitutively activated
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2009; 297(2): R323 - R329.
[Abstract] [Full Text] [PDF]


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