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Am J Physiol Renal Physiol 297: F114-F124, 2009. First published April 29, 2009; doi:10.1152/ajprenal.90752.2008
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Pressure activates epidermal growth factor receptor leading to the induction of iNOS via NF{kappa}B and STAT3 in human proximal tubule cells

Nalini V. Broadbelt,1 Jie Chen,1 Randi B. Silver,2 Dix P. Poppas,1 and Diane Felsen1

1Institute for Pediatric Urology, Department of Urology, and 2Department of Physiology and Biophysics, Weill Cornell Medical Center, New York, New York

Submitted 16 December 2008 ; accepted in final form 27 April 2009

Ureteral obstruction leads to increased pressure and inducible nitric oxide synthase (iNOS) expression. This study examined the involvement of epidermal growth factor (EGF) receptor (EGFR), nuclear factor-{kappa}B (NF{kappa}B), and signal transducers and activators of transcription 3 (STAT3) in iNOS induction in human proximal tubule (HKC-8) cells in response to pressure or EGF. HKC-8 cells were subjected to 60 mmHg pressure or treated with EGF for 0–36 h. iNOS was more rapidly induced in response to EGF than pressure. The addition of EGFR, NF{kappa}B, and STAT3 inhibitors significantly suppressed pressure- or EGF-stimulated iNOS mRNA and protein expression. Analysis of the activated states of EGFR, NF{kappa}B p65, and STAT3 after exposure to both stimuli demonstrated phosphorylation within 2.5 min. Anti-EGF antibody inhibited iNOS induction in pressurized HKC-8 cells, providing evidence that endogenous EGF mediates the response to pressure. In ureteral obstruction, when pressure is elevated, phosphorylated EGFR was detected in the apical surface of the renal tubules, validating the in vitro findings. These data indicate that EGFR, NF{kappa}B, and STAT3 are required for human iNOS gene induction in response to pressure or EGF, indicating a similar mechanism of activation.

cytokine mix; cycloheximide


Address for reprint requests and other correspondence: D. Felsen, Institute for Pediatric Urology, Dept. of Urology, Weill Cornell Medical Center, New York, NY 10021 (e-mail: dfelsen{at}med.cornell.edu)






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