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Am J Physiol Renal Physiol 297: F1006-F1016, 2009. First published July 29, 2009; doi:10.1152/ajprenal.00200.2009
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Decreased abundance of urinary exosomal aquaporin-1 in renal ischemia-reperfusion injury

Hiroko Sonoda,1 Naoko Yokota-Ikeda,2 Sayaka Oshikawa,1 Yosuke Kanno,1 Kazuya Yoshinaga,3 Kazuyuki Uchida,4 Yuuji Ueda,5 Kouichi Kimiya,6 Shigehiro Uezono,2 Akira Ueda,2 Katsuaki Ito,1 and Masahiro Ikeda1

Departments of 1Veterinary Pharmacology and ; 4Veterinary Pathology, Faculty of Agriculture, and ; 3Division of Molecular and Cellular Biology, Department of Anatomy, Faculty of Medicine, University of Miyazaki, Miyazaki; and ; Departments of 2Internal Medicine, ; 5Surgery, and ; 6Urology, Miyazaki Prefectural Miyazaki Hospital, Miyazaki, Japan

Submitted April 9, 2009 ; accepted in final form July 21, 2009

Urinary exosomes, secreted into urine from renal epithelial cells, are known to contain many types of renal functional membrane proteins. Here, we studied whether renal ischemia-reperfusion (I/R) affects urinary exosomal aquaporin-1 (AQP1) excretion in rats subjected to renal I/R and patients who underwent renal transplantation. Immunoblotting studies demonstrated reduction of the urinary exosomal AQP1 level even at 6 h after renal I/R, and the level continued to be low over 96 h after I/R. Renal AQP1 mRNA and protein analyses revealed that the decreased excretion of urinary exosomal AQP1 is associated with renal AQP1 protein retention in the early phase and with a decreased expression level of renal AQP1 in the later phase of renal I/R injury. Decreased abundance of urinary exosomal AQP1 in a recipient patient was also observed at 48 h after renal allograft transplantation. No significant decrease in urinary exosomal AQP1 was observed in a rat model of nephropathy or in patients with proteinuria. Our studies suggest that the renal AQP1 expression level is possibly controlled by its urinary exosomal excretion and indicate that urinary exosomal AQP1 is a novel urinary biomarker for renal I/R injury.

exosomes; renal transplantation


Address for reprint requests and other correspondence: M. Ikeda, Dept. of Veterinary Pharmacology, Faculty of Agriculture, Univ. of Miyazaki, Gakuenkibanadai-nishi 1-1, Miyazaki 889-2192, Japan (e-mail: a0d302u{at}cc.miyazaki-u.ac.jp).






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