Supplemental Data

Files in this Data Supplement:

• Video 1: Dynamics of CD2AP spots on vesicles - GFP-CD2AP transfected podocyte imaged by confocal laser scanning microscopy over 3 min. Video consists of 10 images displayed with 4 frames per second (time compression: 1.3 min real time per 1 s video time). The asterisks marks a vesicle (diameter about 1.5 µm) that probably moves due to a propulsive force generated by the CD2AP spot. Image width represents 40 µm.
• Video 2: Dynamics of CD2AP spots on vesicles. - GFP-CD2AP transfected podocyte imaged by confocal laser scanning microscopy over 3.3 min. Video consists of 11 images displayed with 4 frames per second (time compression: 1.3 min real time per 1 s video time). CD2AP spots form and move on the circumference of three vesicles. Image width represents 18 µm.
• Video 3: Association of CD2AP spots with dextran-filled vesicles - GFP-CD2AP transfected podocyte incubated for 2 h with Alexa546-conjugated dextran (10 kDa) imaged by confocal microscopy over 5.7 min. Video consists of 18 images displayed with 8 frames per second (time compression: 2.7 min real time per 1 s video time). Most of the vesicles associated with CD2AP spots are loaded with dextran after 2 h. Image width represents 40 µm.
• Video 4: Association of CD2AP spots with dextran-filled vesicles - GFP-CD2AP transfected podocyte incubated for 30 min with Alexa546-conjugated dextran 10 kDa imaged by confocal microscopy over 11 min. Video consists of 34 images displayed with 8 frames per second (time compression: 2.7 min real time per 1 s video time). Image width represents 40 µm.
• Video 5: Movement of CD2AP spots depends on actin polymerization - GFP-CD2AP transfected podocyte imaged by confocal laser scanning microscopy over 3.3 min. Video consists of 21 images displayed with 4 frames per second (time compression: 0.7 min real time per 1 s video time). Image collection was resumed 2 min after addition of japlakinolide (1 µM). Image width represents 5 µm.