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Fig. 8.
ETA and
ETB receptors promote mitogenesis
in human mesangial cells. Quiescent cells were stimulated for 48 h with
readdition of agonists (ET-1 and S6c at 100 nM) and antagonists (BQ-610
and PD-145065 at 100 nM) at 24 h. Cells were preincubated with ET
receptor antagonists for 2 min before addition of ET-1 or S6c.
Bromodeoxyuridine (BrdU) was present throughout the incubation, and
BrdU incorporation into DNA was used to assess mitogenesis as described
in MATERIALS AND METHODS and Ref. 40.
Each bar represents >400 cells analyzed from 2 independent
experiments. ** P < 0.01 vs.
ET-1 alone by 
2 analysis.
