Corrigendum
for Ortiz et al., Am J Physiol Renal Physiol 287 (2) F274-F280.
Corrigendum
for Ortiz et al., Am J Physiol Renal Physiol 287 (2) F281-F288.
Am J Physiol Renal Physiol 288: F867-F869, 2005;
doi:10.1152/ajprenal.00010.2005
0363-6127/05 $8.00
CORRIGENDUM
Volume 287, August 2004
Volume 56, August 2004
Pages F274–F280: "Luminal flow induces eNOS activation and translocation in the rat thick ascending limb." Ortiz PA, Hong NJ, and Garvin JL.
Pages F281–F288: "Luminal flow induces eNOS activation and translocation in the rat thick ascending limb. II. Role of PI3-kinase and Hsp90." Ortiz PA, Hong NJ, and Garvin JL.
In the first companion paper noted above, Figure 3, B and C, contains data that reappear in Figure 5, A and C. The corrected figure panels, legend, and pertinent text are shown below.
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Fig. 5. Cytochalasin D blocks flow-induced eNOS translocation in isolated THALs. A: representative confocal micrograph of eNOS localization in an isolated THAL after 30 min of luminal flow (FLOW; scale bar = 10 µm). C: effect of luminal flow on relative amounts of eNOS immunolabeling in the basolateral membrane, the middle of the cell, and the apical membrane of THALs in the absence and presence of cytochalasin D (*P < 0.05 vs. Flow).
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In the text, on p. F276, left column, first full paragraph, the second and third sentences should now read as follows: "We found that preincubation of THALs with cytochalasin D (10 µM) blocked flow-induced eNOS translocation to the apical membrane (flow: apical = 59 ± 2% vs. flow+cytochalsin D: apical = 26 ± 2%, n = 6). The amount of eNOS in the cytoplasm did not change (flow: middle = 25 ± 2% vs. flow+cytochalasin D: middle = 25 ± 1%, n = 6), whereas the amount of eNOS in the basolateral membrane was increased from 17 ± 2% to 49 ± 3% (n = 6, P < 0.05 vs. flow) (Fig. 5)."
In the second companion paper noted above, the control trace in Figure 1A is the same as the trace found in Figure 1A in the first companion paper. Next, Figures 2A and 4A are the same as Figure 3B in the first companion paper. And, finally, the mean data in Figures 2C and 4C are the same data as in Figures 3C and 5C in the first companion paper. The corrected figure panels, legends, and pertinent text are shown below.
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Fig. 1. Phosphatidylinositol-3 kinase (PI3-kinase) inhibition blocks flow-induced nitric oxide (NO) release by isolated thick ascending limbs of Henle's loop (THALs). A: representative traces of flow-induced NO production by isolated thick ascending limbs in the absence and presence of the PI3-kinase inhibitor wortmannin (150 nM).
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Fig. 2. PI3-kinase inhibition blocks flow-induced endothelial nitric oxide synthase (eNOS) translocation to the apical membrane in THALs. A: representative confocal micrograph of eNOS localization in an isolated THAL during luminal flow. C: relative amounts of eNOS immunolabeling in the basolateral membrane, the middle of the cell, and the apical membrane of THALs (*P < 0.05 vs. No flow, #P < 0.05 vs. Flow).
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Fig. 4. Heat shock protein 90 (Hsp90) inhibition blocks flow-induced eNOS translocation to the apical membrane in THALs. A: representative confocal micrograph of eNOS localization in an isolated THAL in the presence of luminal flow (scale bar = 10 µm). C: relative amounts of eNOS immunolabeling in the basolateral membrane, the middle of the cell, and the apical membrane of THALs (*P < 0.05 vs. Flow).
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In the text, on p. F282, right column, first full paragraph, the second and third sentences should now read as follows: "In the absence of flow, eNOS was diffusely distributed throughout the cell cytoplasm and apical and basolateral membranes (basolateral membrane = 39 ± 4%, middle = 37 ± 2%, apical membrane = 24 ± 2% of total fluorescence intensity (Fig. 2C). In the presence of luminal flow, eNOS translocated to the apical membrane (basolateral membrane = 14 ± 1%, middle = 26 ± 2%, apical membrane = 60 ± 1%). Preincubation of THALs with wortmannin (150 nM) completely blocked flow-induced eNOS translocation to the apical membrane (basolateral membrane = 32 ± 3%, middle = 38 ± 1%, apical membrane = 30 ± 1%, n = 5)."
In the text, on p. F283, right column, first full paragraph, the third sentence should now read as follows: "We found that preincubation of THALs with geldanamycin (1 µM) completely blocked flow-induced eNOS translocation to the apical membrane (flow = 60 ± 2%; flow+geldanamycin = 36 ± 1%; *P, 0.05 vs. flow) (Fig. 4C)."
The authors, Editor, and APS staff apologize for the fact that the duplication of data was not recognized during manuscript preparation, peer review, and production.
Copyright © 2005 by the American Physiological Society.
