Am J Physiol Renal Physiol 289: F835-F849, 2005;
doi:10.1152/ajprenal.00122.2005
pripka{at}the-aps.org
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.
Fig. 4. Zebrafish Ae2 mediates Cl–/Cl– exchange. A: 36Cl– uptake into oocytes previously injected with water or with 3 or 10 ng cRNA encoding Ae2 or mAE2a. B: time course of 36Cl– efflux from representative oocytes previously injected with water (
) or with cRNA encoding Ae2 (
) or mAE2a (
). Bath pH was 7.4 in the presence (ND-96) and absence of Cl–. C: 36Cl– efflux rate constants of oocytes expressing Ae2 or mAE2a measured sequentially in ND-96, Cl–-free medium, and finally in ND-96 containing 200 µM DIDS. D: DIDS concentration dependence of 36Cl– efflux rate constants in oocytes expressing Ae2 compared with mAE2a. Values in all bar graphs are means ± SE for (n) oocytes. Oocytes in B–D were injected with 2.7 ng ae2 cRNA or 3 ng of mAE2a cRNA. 36Cl– efflux rate constants in bar graphs reflect subtraction of values measured in water-injected oocytes.
Copyright © 2005 by the American Physiological Society.
