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Am J Physiol Renal Physiol 289: F835-F849, 2005; doi:10.1152/ajprenal.00122.2005

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Table 1. Exon-intron boundaries of the zebrafish ae2 gene

Exon
Intron
Exon

No. Size (nt) 3'-Junction 5'-Junction No. Size: (nt) Z M/R 3'-Junction 5'-Junction No.

1 >145 ACATGACAG gtgagttctt 1 >13495 2088 gtcacatcag GTTATGCTG 2

$~$ 2060

2 159 CAT TTG gtaggtaatc 2 10553 1783 ttacttgtag CCT ATT 3

19-His Leu 1805 Pro Ile-22

3 157 TTT GAG T gtaagagcct 3 3737 125 gtttttttag AT CAT CGT 4

71-Phe Glu T 111 yr His Arg-75

4 257 GCA CAG gtacacacac 4 2267 82 tggcctgcag TTT TTT 5

157-Ala Gln 85 Phe Phe-160

5 125 GGA GCC TC gtgagtgtgc 5 78 1059 atgaccacag G GGC AGA 6

198-Gly Ala Se 1089 r Gly Arg-202

6 233 ATT AAG A gtgagtttgt 6 2091 81 gttgtcgcag GT CAC AGA 7

276-Ile Lys S 87 er His Arg-280

7 140 CAT GAG gtgagaagcc 7 4271 1097 tctctctcag GTT TTT 8

323-His Glu $~$ 1880 Val Phe-326

8 181 TCA CAT G gtgagagaga 8 1233 1161 gattttacag GT GCG GTG 9

383-Ser His G $~$ 1220 ly Ala Val-387

9 136 AAA CAC AG gtcagtttta 9 91 98 tgtttgacag T CAT CCG 10

428-Lys His Se 99 r His Pro-432

10 193 AAC GAG gtattgtgtt 10 301 131 atttctacag AAA GAC 11

493-Asn Glu 132 Lys Asp-496

11 106 CTT GTT G gtcagtttgc 11 1032 83 attttcgcag GC TGT GTG 12

528-Leu Val G 112 ly Cys Val-532

12 185 GAT AAA gtaagcacaa 12 1146 97 tcccacccag CAC TTC 13

590-Asp Lys 111 His Phe-593

13 217 GAC ATC G gtacaaggct 13 102 204 atgttgtcag CT TTA CAA 14

662-Asp Ile A 230 la Leu Gln-666

14 204 CTA TTG G gtttgtttta 14 1625 81 tcttgcacag GT GAG AAG 15

730-Leu Leu G 79 ly Glu Lys-734

15 149 TAT TCA gtaagtttat 15 513 86 gtgtttacag TTT TGC 16

780-Tyr Ser 85 Phe Cys-783

16 195 GGC AAA gtatacaaat 16 2559 2422 tacaccacag ATC TTC 17

845-Gly Lys 2280 Ile Phe-848

17 213 GGC AGG gtgagccagt 17 104 104 gtttgctcag CTG CGA 18

916-Gly Arg 111 Leu Arg-919

18 90 ACA CAG gtgaggaaat 18 2351 86 ctccatatag AAA CTG 19

946-Thr Gln 114 Lys Leu-949

19 167 ATC ACC AC gtgagtcaga 19 2118 338 tgactgtcag T TTG ATA 20

1001-Ile Thr Th 357 r Leu Ile-1005

20 254 CTA GTA G gtgagcggat 20 1403 90 atctccacag GC CTC TCA 21

1086-Leu Val G 91 ly Leu Ser-1090

21 170 CGG AAG gttagtcact 21 3587 153 tttttttcag GTG CGA 22

1143-Arg Lys 154 Val Arg-1146

22 174 CAA TGT gtaagtgatt 22 2094 94 tctgccacag CTC GAT 23

1201-Gln Cys 91 Leu Asp-1204

23 2773 AATAAAGTAATGCTGATTTT

Zebrafish genomic DNA was found as BACs BX663489, BX323804, and CR759864. Intron 1 length remains uncertain because BAC BX663489 lacks exon 1 sequence; BAC BX323804 includes only exon 1 as exonic sequence; BAC CR759864 is currently listed as unordered fragments, but these may encompass intron 1. Intron sizes (nt) are presented for zebrafish (Z), mouse (M, upper italicized number), and rat (R, lower number).

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Exon			Intron					Exon
No.	Size (nt)	3'-Junction	5'-Junction	No.	Size: (nt) Z	M/R	3'-Junction	5'-Junction	No.

1	>145	ACATGACAG	gtgagttctt	1	>13495	2088	gtcacatcag	GTTATGCTG	2
						$~$ 2060
2	159	CAT TTG	gtaggtaatc	2	10553	1783	ttacttgtag	CCT ATT	3
		19-His Leu				1805		Pro Ile-22
3	157	TTT GAG T	gtaagagcct	3	3737	125	gtttttttag	AT CAT CGT	4
		71-Phe Glu T				111		yr His Arg-75
4	257	GCA CAG	gtacacacac	4	2267	82	tggcctgcag	TTT TTT	5
		157-Ala Gln				85		Phe Phe-160
5	125	GGA GCC TC	gtgagtgtgc	5	78	1059	atgaccacag	G GGC AGA	6
		198-Gly Ala Se				1089		r Gly Arg-202
6	233	ATT AAG A	gtgagtttgt	6	2091	81	gttgtcgcag	GT CAC AGA	7
		276-Ile Lys S				87		er His Arg-280
7	140	CAT GAG	gtgagaagcc	7	4271	1097	tctctctcag	GTT TTT	8
		323-His Glu				$~$ 1880		Val Phe-326
8	181	TCA CAT G	gtgagagaga	8	1233	1161	gattttacag	GT GCG GTG	9
		383-Ser His G				$~$ 1220		ly Ala Val-387
9	136	AAA CAC AG	gtcagtttta	9	91	98	tgtttgacag	T CAT CCG	10
		428-Lys His Se				99		r His Pro-432
10	193	AAC GAG	gtattgtgtt	10	301	131	atttctacag	AAA GAC	11
		493-Asn Glu				132		Lys Asp-496
11	106	CTT GTT G	gtcagtttgc	11	1032	83	attttcgcag	GC TGT GTG	12
		528-Leu Val G				112		ly Cys Val-532
12	185	GAT AAA	gtaagcacaa	12	1146	97	tcccacccag	CAC TTC	13
		590-Asp Lys				111		His Phe-593
13	217	GAC ATC G	gtacaaggct	13	102	204	atgttgtcag	CT TTA CAA	14
		662-Asp Ile A				230		la Leu Gln-666
14	204	CTA TTG G	gtttgtttta	14	1625	81	tcttgcacag	GT GAG AAG	15
		730-Leu Leu G				79		ly Glu Lys-734
15	149	TAT TCA	gtaagtttat	15	513	86	gtgtttacag	TTT TGC	16
		780-Tyr Ser				85		Phe Cys-783
16	195	GGC AAA	gtatacaaat	16	2559	2422	tacaccacag	ATC TTC	17
		845-Gly Lys				2280		Ile Phe-848
17	213	GGC AGG	gtgagccagt	17	104	104	gtttgctcag	CTG CGA	18
		916-Gly Arg				111		Leu Arg-919
18	90	ACA CAG	gtgaggaaat	18	2351	86	ctccatatag	AAA CTG	19
		946-Thr Gln				114		Lys Leu-949
19	167	ATC ACC AC	gtgagtcaga	19	2118	338	tgactgtcag	T TTG ATA	20
		1001-Ile Thr Th				357		r Leu Ile-1005
20	254	CTA GTA G	gtgagcggat	20	1403	90	atctccacag	GC CTC TCA	21
		1086-Leu Val G				91		ly Leu Ser-1090
21	170	CGG AAG	gttagtcact	21	3587	153	tttttttcag	GTG CGA	22
		1143-Arg Lys				154		Val Arg-1146
22	174	CAA TGT	gtaagtgatt	22	2094	94	tctgccacag	CTC GAT	23
		1201-Gln Cys				91		Leu Asp-1204
23	2773	AATAAAGTAATGCTGATTTT