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LETTER TO THE EDITOR Dear Editors:

Concerning the comments by Katavetin et al. (1), we recognize that the sense primer used to amplify rat angiotensinogen mRNA is against an intron, reported as intron D of the rat angiotensinogen gene. As pointed out, the amplification happened probably due to a contamination with genomic DNA, although the RT samples were treated with DNAse.

After taking note of the comments by Katavetin et al. (1) (by searching Medline), we designed new primers and the experiments were redone. The results were coincident to those presented in the original paper (3); i.e., high glucose concentration induced an approximately twofold increase in the angiotensinogen mRNA (0.98 ± 0.06 in control cells vs. 1.98 ± 0.5 in glucose stimulated cells). This result was also described by others (2) and had been previously found in our laboratory by Northern blot analysis. Therefore, we understand that this mistake (see also the corrigendum that follows) does not invalidate our conclusion.

The correct primer sequences for amplification of angiotensinogen mRNA were the following: sense, 5'-tggaagcagcagccagacac-3'; and antisense, 5'-ggcagcaagaactgggtcag-3'.

REFERENCES

1. Katavetin P, Nagaku M, and Fugita T. Wrong primers for rat angiotensinogen mRNA. Am J Physiol Renal Physiol 288: F1078, 2005.[Free Full Text]
2. Singh R, Singh AK, Alavi N, and Leehey DJ. Mechanism of increased angiotensin II levels in glomerular mesangial cells cultured in high glucose. J Am Soc Nephrol 14: 873–880, 2003.[Abstract/Free Full Text]
3. Vidotti DB, Casarini DE, Christovam PC, Leite CA, Schor N, and Boim MA. High glucose concentration stimulates intracellular renin activity and angiotensin II generation in rat mesangial cells. Am J Physiol Renal Physiol 286: F1039–F1045, 2004.[Abstract/Free Full Text]

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