AJP - Renal Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (June 25, 2008). doi:10.1152/ajprenal.00002.2008
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
295/3/F780    most recent
00002.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Estilo, G.
Right arrow Articles by Satlin, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Estilo, G.
Right arrow Articles by Satlin, L. M.
Submitted on January 3, 2008
Accepted on June 19, 2008

Effect of aldosterone on BK channel expression in the mammalian cortical collecting duct (CCD)

Genevieve Estilo1, Wen Liu2, Nuria Pastor-Soler3, Phillip Mitchell2, Marcelo Daniel Carattino3, Thomas R Kleyman3, and Lisa M. Satlin4*

1 Medicine, Mount Sinai School of Medicine, New York, New York, United States
2 Pediatrics, Mount Sinai School of Medicine, New York, New York, United States
3 Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, United States
4 Department of Pediatrics, Box 1664, Mount Sinai School of Medicine, New York, New York, United States; , United States

* To whom correspondence should be addressed. E-mail: ajprenal{at}emory.edu.

Apical BK channels in the CCD mediate flow-stimulated K secretion. Dietary K loading for 10-14 d leads to an increase in BK channel mRNA abundance, enhanced flow-stimulated K secretion in microperfused CCDs, and a redistribution of immunodetectable channels from an intracellular pool to the apical membrane (Najjar et al, AJP Renal 289:F922, 2005). To test whether this adaptation was mediated by a K-induced increase in aldosterone, NZW rabbits were fed a low (LS) or high (HS) Na diet for 7-10 d to alter circulating levels of aldosterone but not serum K concentration. Single CCDs were isolated for quantitation of BK channel subunit (total, {alpha} splice variants, {beta} isoforms) mRNA abundance by real-time PCR and measurement of net transepithelial Na (JNa) and K (JK) transport by microperfusion; kidneys were processed for immunolocalization of BK {alpha} subunit by immunofluorescence microscopy. At the time of sacrifice, LS rabbits excreted no urinary Na and had higher circulating levels of aldosterone than did HS animals. The relative abundance of BK {alpha}, {beta}2, and {beta}4-subunit mRNA and localization of immunodetectable {alpha} subunit were similar in CCDs from LS and HS animals. In response to an increase in tubular flow rate from 1 to 5 nl/min per mm, the increase in JNa tended to be greater in LS vs. HS rabbits, yet the flow-stimulated increase in JK was similar in both groups. These data suggest that aldosterone does not contribute to the regulation of BK channel expression/activity in response to dietary K loading.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Renal Physiol.Home page
W. Liu, Y. Wei, P. Sun, W.-H. Wang, T. R. Kleyman, and L. M. Satlin
Mechanoregulation of BK channel activity in the mammalian cortical collecting duct: role of protein kinases A and C
Am J Physiol Renal Physiol, October 1, 2009; 297(4): F904 - F915.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
P. R. Grimm, D. L. Irsik, L. Liu, J. D. Holtzclaw, and S. C. Sansom
Role of BK{beta}1 in Na+ reabsorption by cortical collecting ducts of Na+-deprived mice
Am J Physiol Renal Physiol, August 1, 2009; 297(2): F420 - F428.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. R. Grimm, D. L. Irsik, D. C. Settles, J. D. Holtzclaw, and Steven. C. Sansom
Hypertension of Kcnmb1-/- is linked to deficient K secretion and aldosteronism
PNAS, July 14, 2009; 106(28): 11800 - 11805.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.