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Am J Physiol Renal Physiol (May 6, 2003). doi:10.1152/ajprenal.00013.2003
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Submitted on January 13, 2003
Accepted on April 8, 2003

Effect of the Isolated Removal of Either Basolateral HCO-3 or Basolateral CO2 on HCO-3 Reabsorption by the Rabbit S2 Proximal Tubule

Jinhua Zhao1, Yuehan Zhou1, and Walter F. Boron1*

1 Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA

* To whom correspondence should be addressed. E-mail: walter.boron{at}yale.edu.

The equilibrium CO2 + H2O {leftrightarrows} H+ + HCO-3, had made it impossible to determine how isolated changes in basolateral [CO2] or [HCO-3]__at a fixed basolateral pH__modulate renal HCO-3 reabsorption. In the present study, we have begun to address this issue by measuring HCO-3 reabsorption (JHCO3) and intracellular pH (pHi) in isolated perfused rabbit S2 proximal tubules exposed to three different basolateral (bath) solutions: (i) equilibrated 5% CO2/22 mM HCO-3/pH 7.40, (ii) an out-of-equilibrium (OOE) solution containing 5% CO2/pH 7.40 but minimal HCO-3 ("pure CO2"), and (iii) an OOE solution containing 22 mM HCO-3/pH 7.40 but minimal CO2 ("pure HCO-3"). Tubule lumens were constantly perfused with equilibrated 5% CO2/22 mM HCO-3. Compared to the equilibrated bath solution (JHCO3 = 76.5 ± 7.7.pmole.min-1.mm-1, pHi = 7.09 ± 0.04), the "pure CO2" bath solution increased JHCO3 by ~25% but decreased pHi by 0.19. In contrast, the "pure HCO-3" bath solution decreased JHCO3 by 37% but increased pHi by 0.24. Our data are consistent with two competing hypotheses: (i) The isolated removal of basolateral HCO-3 (or CO2) causes a pHi decrease (increase) that in turn raises (lowers) JHCO3. (ii) HCO-3 removal raises JHCO3 by reducing inhibition of basolateral Na/HCO3 cotransport and/or reducing HCO-3 backleak, whereas CO2 removal lowers JHCO3 by reducing stimulation of a CO2 sensor.




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