AJP - Renal AJP: Gastrointestinal and Liver Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (May 24, 2005). doi:10.1152/ajprenal.00019.2005
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
289/4/F891    most recent
00019.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jernigan, N. L.
Right arrow Articles by Drummond, H. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jernigan, N. L.
Right arrow Articles by Drummond, H. A.
Submitted on January 19, 2005
Accepted on May 20, 2005

Vascular ENaC Proteins are Required for Renal Myogenic Constriction

Nikki L. Jernigan1 and Heather A. Drummond1*

1 Department of Physiology and Biophysics and the Center for Excellence in Caediovascular Renal Research, University of Mississippi Medical Center, Jackson, MS, USA

* To whom correspondence should be addressed. E-mail: hdrummond{at}physiology.umsmed.edu.

The myogenic response is an essential component of renal blood flow autoregulation and is the inherent ability of vascular smooth muscle cells (VSMCs) to contract in response to increases in intraluminal pressure. Although mechanosensitive ion channels are thought to initiate VSMC stretch-induced contraction, their molecular identity is unknown. Recent reports suggest Degenerin/Epithelial Na+ Channels (DEG/ENaC) may form mechanotransducers in sensory neurons and VSMCs, however, the role of DEG/ENaC proteins in myogenic constriction of mouse renal arteries has not been established. To test the hypothesis DEG/ENaC proteins are required for myogenic constriction in renal vessels, we first determined expression of ENaC transcripts and proteins in mouse renal VSMCs (mrVSMCs). Then, we determined pressure- and agonist-induced constriction and changes in VSM cytosolic Ca2+ and Na+ in isolated mouse renal interlobar arteries following DEG/ENaC inhibition with amiloride and benzamil. We detect {alpha}-, {beta}-, and {gamma}ENaC transcript and protein expression in cultured mrVSMC. In contrast, we detect only {beta}- and {gamma}-, but not {alpha}-, ENaC protein in freshly dispersed mrVMSC. Selective DEG/ENaC inhibition, with low doses of amiloride and benzamil, abolish pressure-induced constriction and increases in cytosolic Ca2+ and Na+ without diminishing agonist-induced responses in isolated mouse interlobar arteries. Our findings indicate DEG/ENaC proteins are required for myogenic constriction in mouse interlobar arteries and are consistent with our hypothesis that DEG/ENaC proteins may be components of mechanosensitive ion channel complexes required for myogenic vasoconstriction.




This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
K. Khavandi, A. S. Greenstein, K. Sonoyama, S. Withers, A. Price, R. A. Malik, and A. M. Heagerty
Myogenic tone and small artery remodelling: insight into diabetic nephropathy
Nephrol. Dial. Transplant., November 21, 2008; (2008) gfn583v1.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Wang, K. Takeya, P. I. Aaronson, K. Loutzenhiser, and R. Loutzenhiser
Effects of amiloride, benzamil, and alterations in extracellular Na+ on the rat afferent arteriole and its myogenic response
Am J Physiol Renal Physiol, July 1, 2008; 295(1): F272 - F282.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
N. Kleinstreuer, T. David, M. J. Plank, and Z. Endre
Dynamic myogenic autoregulation in the rat kidney: a whole-organ model
Am J Physiol Renal Physiol, June 1, 2008; 294(6): F1453 - F1464.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. C. Grifoni, S. E. McKey, and H. A. Drummond
Hsc70 regulates cell surface ASIC2 expression and vascular smooth muscle cell migration
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2022 - H2030.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
H. A. Drummond, N. L. Jernigan, and S. C. Grifoni
Sensing Tension: Epithelial Sodium Channel/Acid-Sensing Ion Channel Proteins in Cardiovascular Homeostasis
Hypertension, May 1, 2008; 51(5): 1265 - 1271.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. P. Gannon, L. G. VanLandingham, N. L. Jernigan, S. C. Grifoni, G. Hamilton, and H. A. Drummond
Impaired pressure-induced constriction in mouse middle cerebral arteries of ASIC2 knockout mice
Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1793 - H1803.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
H. A. Drummond, S. C. Grifoni, and N. L. Jernigan
A New Trick for an Old Dogma: ENaC Proteins as Mechanotransducers in Vascular Smooth Muscle
Physiology, February 1, 2008; 23(1): 23 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. Schubert, D. Lidington, and S.-S. Bolz
The emerging role of Ca2+ sensitivity regulation in promoting myogenic vasoconstriction
Cardiovasc Res, January 1, 2008; 77(1): 8 - 18.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
N. L. Jernigan, B. LaMarca, J. Speed, L. Galmiche, J. P. Granger, and H. A. Drummond
Dietary salt enhances benzamil-sensitive component of myogenic constriction in mesenteric arteries
Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H409 - H420.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. D. Carattino, W. Liu, W. G. Hill, L. M. Satlin, and T. R. Kleyman
Lack of a role of membrane-protein interactions in flow-dependent activation of ENaC
Am J Physiol Renal Physiol, July 1, 2007; 293(1): F316 - F324.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. C. Grifoni, K. P. Gannon, D. E. Stec, and H. A. Drummond
ENaC proteins contribute to VSMC migration
Am J Physiol Heart Circ Physiol, December 1, 2006; 291(6): H3076 - H3086.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. R. Cao, P. P. Shi, R. D. Sigmund, R. F. Husted, C. D. Sigmund, R. A. Williamson, J. B. Stokes, and B. Yang
Mice heterozygous for beta-ENaC deletion have defective potassium excretion
Am J Physiol Renal Physiol, July 1, 2006; 291(1): F107 - F115.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. S. Amin, H.-W. Wang, E. Reza, S. C. Whitman, B. S. Tuana, and F. H. H. Leenen
Distribution of epithelial sodium channels and mineralocorticoid receptors in cardiovascular regulatory centers in rat brain
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2005; 289(6): R1787 - R1797.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.