AJP - Renal Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (June 25, 2008). doi:10.1152/ajprenal.00019.2008
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
295/3/F772    most recent
00019.2008v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (4)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gonzalez-Villalobos, R. A.
Right arrow Articles by Navar, L. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gonzalez-Villalobos, R. A.
Right arrow Articles by Navar, L. G.
Submitted on January 11, 2008
Accepted on June 19, 2008

INTRARENAL ANGIOTENSIN II AND ANGIOTENSINOGEN AUGMENTATION IN CHRONIC ANGIOTENSIN II-INFUSED MICE

Romer A. Gonzalez-Villalobos1*, Dale M Seth1, Ryousuke Satou1, Heather Horton1, Naro Ohashi1, Kayoko Miyata1, Akemi Katsurada1, Duy V Tran1, Hiroyuki Kobori1, and L. Gabriel Navar1

1 Department of Physiology and Hypertension and Renal Center of Excellence, Tulane University Health Sciences Center, New Orleans, Louisiana, United States

* To whom correspondence should be addressed. E-mail: rgonzale{at}tulane.edu.

The objectives of this study were to determine the effects of chronic angiotensin II infusions on angiotensin II content and angiotensinogen expression in the mouse kidney and the role of the angiotensin II type 1 receptor (AT1R) in mediating these changes. C57BL/6J male mice were subjected to angiotensin II infusions at doses of 400 or 1000 ng/kg/min either alone or with an AT1R blocker (Olmesartan 3 mg/kg/d) for 12 days. Systolic and mean arterial pressures (SBP and MAP) were determined by tail-cuff plethysmography and radiotelemetry. On day 13, blood and kidneys were collected for angiotensin II determinations by radioimmunoanalysis and intrarenal angiotensinogen expression studies by qRT-PCR, Western blot and immunohistochemistry. Angiotensin II infusions at the low dose elicited progressive increases in SBP (135±2.5 mmHg). In contrast, the high dose induced a rapid increase (152±2.5) (p<0.05 versus controls, 109±2.8). Renal angiotensin II content was increased by angiotensin II infusions at the low dose (1203±253 fmol/g) and the high dose (1258±173) versus controls (499±40, p<0.05). Kidney angiotensinogen mRNA and protein were increased only by the low dose to 1.13±0.02 and 1.26±0.10 respectively over controls (1.00, p<0.05). These effects were not observed in mice infused at the high dose and those receiving olmesartan. The results indicate that chronic Ang II infusions augment mouse intrarenal angiotensin II content with AT1R-dependent uptake occurring at both doses, but only the low dose of infusion, which elicited a slow progressive response, causes an AT1R-dependent increase in intrarenal angiotensinogen expression.




This article has been cited by other articles:


Home page
 HypertensionHome page
D. Zhao, D. M. Seth, and L. G. Navar
Enhanced Distal Nephron Sodium Reabsorption in Chronic Angiotensin II-Infused Mice
Hypertension, July 1, 2009; 54(1): 120 - 126.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
W. Shao, D. M. Seth, and L. G. Navar
Augmentation of endogenous intrarenal angiotensin II levels in Val5-ANG II-infused rats
Am J Physiol Renal Physiol, May 1, 2009; 296(5): F1067 - F1071.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
R. A. Gonzalez-Villalobos, R. Satou, D. M. Seth, L. C. Semprun-Prieto, A. Katsurada, H. Kobori, and L. G. Navar
Angiotensin-Converting Enzyme-Derived Angiotensin II Formation During Angiotensin II-Induced Hypertension
Hypertension, February 1, 2009; 53(2): 351 - 355.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
A. Fekete, K. Rosta, L. Wagner, A. Prokai, P. Degrell, E. Ruzicska, E. Vegh, M. Toth, K. Ronai, K. Rusai, et al.
Na+,K+-ATPase is modulated by angiotensin II in diabetic rat kidney - another reason for diabetic nephropathy?
J. Physiol., November 15, 2008; 586(22): 5337 - 5348.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.