AJP - Renal Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (March 25, 2003). doi:10.1152/ajprenal.00032.2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
285/1/F68    most recent
00032.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bergeron, M.
Right arrow Articles by Isenring, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bergeron, M.
Right arrow Articles by Isenring, P.
Submitted on January 22, 2003
Accepted on March 18, 2003

Ammonium transport and pH regulation by K+-Cl- cotransporters

Marc Bergeron1, Edith Gagnon1, Bernadette Wallendorff2, Jean-Yves Lapointe2, and Paul Isenring1*

1 Department of Medicine, Laval University, Quebec, QC, Canada
2 Department of Physics, Montreal University, Montreal, QC, Canada

* To whom correspondence should be addressed. E-mail: paul.isenring{at}crhdq.ulaval.ca.

The Na+-K+-Cl- cotransporters (NKCCs), which belong to the cation-Cl- cotransporter (CCC) family, are able to translocate NH4+ across cell membranes. In this work, we have used the oocyte expression system to determine whether the K+-Cl- cotransporters (KCCs) can also transport NH4+ and whether they play a role in pH regulation. Our results demonstrate that all of the CCCs examined (NKCC1, NKCC2, KCC1, KCC3, and KCC4) can promote NH4+ translocation, presumably through binding of the ion at the K+ site. Moreover, kinetic studies for both NKCCs and KCCs suggest that NH4+ is an excellent surrogate of Rb+ or K+, and that NH4+ transport and cellular acidification resulting from CCC activity is relevant physiologically. In this study, we have also found that CCCs are strongly and differentially affected by changes in intracellular pH (independently of intracellular [NH4+]). Indeed, NKCC2, KCC1, and KCC3 are inhibited at intracellular pH < 7.5 whereas KCC4 is activated. These results indicate that certain CCC isoforms may be specialized to operate in acidic environments. CCC mediated NH4+ transport could bear great physiological implication given the ubiquitous distribution of these carriers.




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
Y. Kim and L. O. Trussell
Negative Shift in the Glycine Reversal Potential Mediated by a Ca2+- and pH-Dependent Mechanism in Interneurons
J. Neurosci., September 16, 2009; 29(37): 11495 - 11510.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. F. Simard, M. J. Bergeron, R. Frenette-Cotton, G. A. Carpentier, M.-E. Pelchat, L. Caron, and P. Isenring
Homooligomeric and Heterooligomeric Associations between K+-Cl- Cotransporter Isoforms and between K+-Cl- and Na+-K+-Cl- Cotransporters
J. Biol. Chem., June 22, 2007; 282(25): 18083 - 18093.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
B. Yang, D. Zhao, E. Solenov, and A. S. Verkman
Evidence from knockout mice against physiologically significant aquaporin 8-facilitated ammonia transport
Am J Physiol Cell Physiol, September 1, 2006; 291(3): C417 - C423.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
M. E. Handlogten, S.-P. Hong, L. Zhang, A. W. Vander, M. L. Steinbaum, M. Campbell-Thompson, and I. D. Weiner
Expression of the ammonia transporter proteins Rh B glycoprotein and Rh C glycoprotein in the intestinal tract
Am J Physiol Gastrointest Liver Physiol, May 1, 2005; 288(5): G1036 - G1047.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. Gamba
Molecular Physiology and Pathophysiology of Electroneutral Cation-Chloride Cotransporters
Physiol Rev, April 1, 2005; 85(2): 423 - 493.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. R. Williams and J. A. Payne
Cation transport by the neuronal K+-Cl- cotransporter KCC2: thermodynamics and kinetics of alternate transport modes
Am J Physiol Cell Physiol, October 1, 2004; 287(4): C919 - C931.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. E. Handlogten, S.-P. Hong, C. M. Westhoff, and I. D. Weiner
Basolateral ammonium transport by the mouse inner medullary collecting duct cell (mIMCD-3)
Am J Physiol Renal Physiol, October 1, 2004; 287(4): F628 - F638.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Gagnon, M. J. Bergeron, G. M. Brunet, N. D. Daigle, C. F. Simard, and P. Isenring
Molecular Mechanisms of Cl- Transport by the Renal Na+-K+-Cl- Cotransporter: IDENTIFICATION OF AN INTRACELLULAR LOCUS THAT MAY FORM PART OF A HIGH AFFINITY Cl--BINDING SITE
J. Biol. Chem., February 13, 2004; 279(7): 5648 - 5654.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
X. Liu, S. Titz, A. Lewen, and U. Misgeld
KCC2 Mediates NH4+ Uptake in Cultured Rat Brain Neurons
J Neurophysiol, October 1, 2003; 90(4): 2785 - 2790.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1976 by the American Physiological Society.