| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
-methyl-D-glucopyranoside uptake in renal proximal tubule cells: Involvement of STAT3, PI3K/Akt, MAPKs, and NF-kB
1 College of Veterinary Medicine, Chonnam National University, Biotherapy Human Resources Center, Gwangju, Korea, Republic of
* To whom correspondence should be addressed. E-mail: hjhan{at}chonnam.ac.kr.
Recent studies have shown that interleukin 6 (IL-6) acts on the cellular proliferation activating transduction signals during cellular regeneration. Therefore, this study examined the effect of IL-6 on the activation of Na+/glucose cotransporters (SGLTs) and its related signaling pathways in primary cultured renal proximal tubule cells (PTCs). IL-6 increased the level of 14C-
-methyl-D-glucopyranoside (-MG) uptake in time and dose dependent manners. IL-6 also increased SGLT1 plus SGLT2 mRNA and protein expression level. The IL-6 receptors [IL-6 subunit (IL-6R) and IL-6 
subunit (gp130)] were expressed in PTCs. In addition, genistein and herbimycin A completely blocked the IL-6-induced increases in -MG uptake and the protein expression level of SGLTs. On the other hand, IL-6 increased the level of 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate (CM-H2DCF-DA)-sensitive cellular reactive oxygen species (ROS) and IL-6-induced increases in -MG uptake and the protein expression level of SGLTs were blocked by ascorbic acid or taurine. IL-6 also increased the phosphorylation of signal transducer and activator of transcription-3 (STAT3), phosphoinositide-3 kinase (PI3K)/Akt, and mitogen-activated protein kinases (MAPKs) in a time dependent manner. A pretreatment with STAT3 inhibitor, LY 294002, Akt inhibitor, PD 98059, SB 203580, or SP 600125 significantly blocked the IL-6-induced increase in -MG uptake. In addition, IL-6 increased the level of nuclear factor-kappa B (NF-kB) phosphorylation. A pretreatment with SN50 or Bay11-7082 also blocked the IL-6-induced increase in -MG uptake. In conclusion, IL-6 increases the Na+/glucose cotransporter activity through reactive oxygen species (ROS), and its action in renal proximal tubule cells is associated with the STAT3, PI3K/Akt, MAPKs, and NF-kB signaling pathways.
This article has been cited by other articles:
|
|
 |
|
  G. Byfield, S. Budd, and M. E. Hartnett The Role of Supplemental Oxygen and JAK/STAT Signaling in Intravitreous Neovascularization in a ROP Rat Model Invest. Ophthalmol. Vis. Sci., July 1, 2009; 50(7): 3360 - 3365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Lee, H. N. Suh, and H. J. Han Effect of BSA-induced ER stress on SGLT protein expression levels and {alpha}-MG uptake in renal proximal tubule cells Am J Physiol Renal Physiol, June 1, 2009; 296(6): F1405 - F1416. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Arakawa, T. Masaki, T. Hirai, S. Doi, M. Kuratsune, K. Arihiro, N. Kohno, and N. Yorioka Activation of signal transducer and activator of transcription 3 correlates with cell proliferation and renal injury in human glomerulonephritis Nephrol. Dial. Transplant., November 1, 2008; 23(11): 3418 - 3426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Satou, R. A. Gonzalez-Villalobos, K. Miyata, N. Ohashi, A. Katsurada, L. G. Navar, and H. Kobori Costimulation with angiotensin II and interleukin 6 augments angiotensinogen expression in cultured human renal proximal tubular cells Am J Physiol Renal Physiol, July 1, 2008; 295(1): F283 - F289. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
