| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
1 Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA; Indiana Center for Biological Microscopy, Indiana University School of Medicine, Indianapolis, IN, USA
2 Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA; Indiana Center for Biological Microscopy, Indiana University School of Medicine, Indianapolis, IN, USA; Roudebush VA Medical Center, Indianapolis, IN, USA
3 Division of Neonatology, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN, USA
* To whom correspondence should be addressed. E-mail: tsutton2{at}iupui.edu.
The role of renal microvascular endothelial cell injury in the pathophysiology of ischemic acute renal failure (ARF) remains largely unknown. No consistent morphologic alterations have been ascribed to the endothelium of the renal microvasculature as a result of ischemia-reperfusion injury. Therefore, the purpose of this study was to examine biochemical markers of endothelial injury and morphologic changes of the renal microvascular endothelium in a rodent model of ischemic ARF. Circulating von Willebrand factor (vWF) was measured as a marker of endothelial injury. Twenty-four hours after ischemia, circulating vWF peaked at 124% over baseline values (p
0.001). The FVB-TIE2/GFP mouse was utilized to localize morphologic changes in the renal microvascular endothelium. Immediately following ischemia, there was a marked increase in F-actin aggregates in the basal and basolateral aspect of renal microvascular endothelial cells in the corticomedullary junction. Following 24 hours of reperfusion, the pattern of F-actin staining was more similar to that observed under physiologic conditions. In addition, alterations in the integrity of the adherens junctions of the renal microvasculature, as demonstrated by loss of localization in VE-cadherin immunostaining, were observed following 24 hours of reperfusion. This observation temporally correlated with the greatest extent of permeability defect in the renal microvasculature as identified using fluorescent dextrans and 2-photon intravital imaging. Taken together, these findings indicate that renal vascular endothelial injury occurs in ischemic ARF and may play an important role in the pathophysiology of ischemic ARF.
This article has been cited by other articles:
|
|
 |
|
  Y. J. Jung, D. H. Kim, A. S. Lee, S. Lee, K. P. Kang, S. Y. Lee, K. Y. Jang, M. J. Sung, S. K. Park, and W. Kim Peritubular capillary preservation with COMP-angiopoietin-1 decreases ischemia-reperfusion-induced acute kidney injury Am J Physiol Renal Physiol, October 1, 2009; 297(4): F952 - F960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wu, R. Guo, P. Chen, Q. Wang, and P. N. Cunningham TNF induces caspase-dependent inflammation in renal endothelial cells through a Rho- and myosin light chain kinase-dependent mechanism Am J Physiol Renal Physiol, August 1, 2009; 297(2): F316 - F326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Legrand, E. Almac, E. G. Mik, T. Johannes, A. Kandil, R. Bezemer, D. Payen, and C. Ince L-NIL prevents renal microvascular hypoxia and increase of renal oxygen consumption after ischemia-reperfusion in rats Am J Physiol Renal Physiol, May 1, 2009; 296(5): F1109 - F1117. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. A. Sharfuddin, R. M. Sandoval, D. T. Berg, G. E. McDougal, S. B. Campos, C. L. Phillips, B. E. Jones, A. Gupta, B. W. Grinnell, and B. A. Molitoris Soluble Thrombomodulin Protects Ischemic Kidneys J. Am. Soc. Nephrol., March 1, 2009; 20(3): 524 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Campos, S. L. Ashworth, S. Wean, M. Hosford, R. M. Sandoval, M. A. Hallett, S. J. Atkinson, and B. A. Molitoris Cytokine-induced F-actin reorganization in endothelial cells involves RhoA activation Am J Physiol Renal Physiol, March 1, 2009; 296(3): F487 - F495. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Leonard, J. L. Friedrich, and D. P. Basile VEGF-121 preserves renal microvessel structure and ameliorates secondary renal disease following acute kidney injury Am J Physiol Renal Physiol, December 1, 2008; 295(6): F1648 - F1657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kwon, S.-M. Hong, T. A. Sutton, and C. J. Temm Preservation of peritubular capillary endothelial integrity and increasing pericytes may be critical to recovery from postischemic acute kidney injury Am J Physiol Renal Physiol, August 1, 2008; 295(2): F351 - F359. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mittalhenkle, C. O. Stehman-Breen, M. G. Shlipak, L. F. Fried, R. Katz, B. A. Young, S. Seliger, D. Gillen, A. B. Newman, B. M. Psaty, et al. Cardiovascular Risk Factors and Incident Acute Renal Failure in Older Adults: The Cardiovascular Health Study Clin. J. Am. Soc. Nephrol., March 1, 2008; 3(2): 450 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. H. Lu, D.-J. Oh, B. Dursun, Z. He, T. S. Hoke, S. Faubel, and C. L. Edelstein Increased Macrophage Infiltration and Fractalkine Expression in Cisplatin-Induced Acute Renal Failure in Mice J. Pharmacol. Exp. Ther., January 1, 2008; 324(1): 111 - 117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-J. Oh, B. Dursun, Z. He, L. Lu, T. S. Hoke, D. Ljubanovic, S. Faubel, and C. L. Edelstein Fractalkine receptor (CX3CR1) inhibition is protective against ischemic acute renal failure in mice Am J Physiol Renal Physiol, January 1, 2008; 294(1): F264 - F271. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Horbelt, S.-Y. Lee, H. E. Mang, N. L. Knipe, Y. Sado, A. Kribben, and T. A. Sutton Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injury Am J Physiol Renal Physiol, September 1, 2007; 293(3): F688 - F695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nitescu, E. Grimberg, S.-E. Ricksten, N. Marcussen, and G. Guron Thrombin inhibition with melagatran does not attenuate renal ischaemia-reperfusion injury in rats Nephrol. Dial. Transplant., August 1, 2007; 22(8): 2149 - 2155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Spurgeon-Pechman, D. L. Donohoe, D. L. Mattson, H. Lund, L. James, and D. P. Basile Recovery from acute renal failure predisposes hypertension and secondary renal disease in response to elevated sodium Am J Physiol Renal Physiol, July 1, 2007; 293(1): F269 - F278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Wu, N. Gokden, and P. R. Mayeux Evidence for the Role of Reactive Nitrogen Species in Polymicrobial Sepsis-Induced Renal Peritubular Capillary Dysfunction and Tubular Injury J. Am. Soc. Nephrol., June 1, 2007; 18(6): 1807 - 1815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yu, R. M. Sandoval, and B. A. Molitoris Rapid determination of renal filtration function using an optical ratiometric imaging approach Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1873 - F1880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Andersson, U. Nilsson, C. Hjalmarsson, B. Haraldsson, and J. S. Nystrom Mild renal ischemia-reperfusion reduces charge and size selectivity of the glomerular barrier Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1802 - F1809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Togel, K. Weiss, Y. Yang, Z. Hu, P. Zhang, and C. Westenfelder Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1626 - F1635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Guan, G. Gobe, D. Willgoss, and Z. H. Endre Renal endothelial dysfunction and impaired autoregulation after ischemia-reperfusion injury result from excess nitric oxide Am J Physiol Renal Physiol, September 1, 2006; 291(3): F619 - F628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Biswas, S. Canosa, D. Schoenfeld, J. Schoenfeld, P. Li, L. C. Cheas, J. Zhang, A. Cordova, B. Sumpio, and J. A. Madri PECAM-1 Affects GSK-3{beta}-Mediated {beta}-Catenin Phosphorylation and Degradation Am. J. Pathol., July 1, 2006; 169(1): 314 - 324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Devarajan Update on Mechanisms of Ischemic Acute Kidney Injury J. Am. Soc. Nephrol., June 1, 2006; 17(6): 1503 - 1520. [Full Text] [PDF]
|
|
|
|
|
|
M. V. Suurna, S. L. Ashworth, M. Hosford, R. M. Sandoval, S. E. Wean, B. M. Shah, J. R. Bamburg, and B. A. Molitoris Cofilin mediates ATP depletion-induced endothelial cell actin alterations Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1398 - F1407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. N. Lee, E. Huang, and H. J. Ward Tight junction biology and kidney dysfunction Am J Physiol Renal Physiol, January 1, 2006; 290(1): F20 - F34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Yu, R. M. Sandoval, and B. A. Molitoris Quantitative intravital microscopy using a Generalized Polarity concept for kidney studies Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1197 - C1208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Xi, X. Chen, X. Sun, S. Shi, Z. Feng, J. Wang, Q. Hong, Y. Lu, and S. Lin Effects of Alterations of Glomerular Fibrin Deposition on Renal Inflammation in Rats at Different Age Stages J. Gerontol. A Biol. Sci. Med. Sci., September 1, 2005; 60(9): 1099 - 1110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hirata, Y. Osuga, O. Yoshino, Y. Hirota, M. Harada, Y. Takemura, C. Morimoto, K. Koga, T. Yano, O. Tsutsumi, et al. Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein Hum. Reprod., August 1, 2005; 20(8): 2092 - 2096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Spurgeon, D. L. Donohoe, and D. P. Basile Transforming growth factor-{beta} in acute renal failure: receptor expression, effects on proliferation, cellularity, and vascularization after recovery from injury Am J Physiol Renal Physiol, March 1, 2005; 288(3): F568 - F577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Sutton, K. J. Kelly, H. E. Mang, Z. Plotkin, R. M. Sandoval, and P. C. Dagher Minocycline reduces renal microvascular leakage in a rat model of ischemic renal injury Am J Physiol Renal Physiol, January 1, 2005; 288(1): F91 - F97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Garwood Renal Insufficiency After Cardiac Surgery Seminars in Cardiothoracic and Vascular Anesthesia, September 1, 2004; 8(3): 227 - 241. [Abstract] [PDF]
|
|
|
|
|
|
D. P. Basile, K. Fredrich, D. Weihrauch, N. Hattan, and W. M. Chilian Angiostatin and matrix metalloprotease expression following ischemic acute renal failure Am J Physiol Renal Physiol, May 1, 2004; 286(5): F893 - F902. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
