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1 Department of Physiology, Box 7-3, LSU Health Sciences Center School of Medicine, New Orleans, Louisiana, United States
* To whom correspondence should be addressed. E-mail: lharris{at}lsuhsc.edu.
Angiotensin type 1A (AT1A) and 1B (AT1B) receptor deletion (AT1DKO) results in renal microvascular disease, tubulointerstitial injury, and reduced blood pressure. To test the hypothesis that renal preglomerular responses to angiotensin (ANG) II are mediated by AT1A and AT1B receptors, experiments were performed in AT1DKO mice using the in vitro blood perfused juxtamedullary nephron technique. Kidneys were harvested from AT1DKO and wild-type (WT) mice and bathed with ANG II (1-100 nM), norepinephrine (NE; 100-1000 nM), or acetylcholine (ACh; 10 µM). Baseline diameters of afferent (19.5 ± 0.7 and 13.9 ± 0.7 µm, n = 17 and 16) and efferent (15.5 ±2.1 and 10.8 ±1.0 µm, n = 4 and 7) arterioles of AT1DKO were significantly larger than WT. Afferent and efferent arteriole responses to ANG II, 100, and 300 nM NE were absent in AT1DKO; although significant constriction to 1 µM NE was observed (-17 ±5 and -23 ± 6 %, respectively). Afferent arterioles of WT mice dilated significantly in response to ACh (15.1 ± 0.6 to 17.0 ± 1.2 µm, n = 6); however, arterioles from AT1DKO tended to contract (19.9 ± 1.2 to 17.8 ± 1.6 µm; n = 6, P = 0.06). In summary, loss of ANG II-induced contraction, reduced vasoconstriction to NE, and endothelial cell dysfunction contribute to the renal vascular phenotype of AT1DKO mice. We conclude that ANG II signaling via the AT1 receptor plays a pivotal role in basal renal microvascular tone and effectiveness to respond to vasoconstrictor and vasodilator agonists.
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