| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 College of Medicine, University of FL, Gainesville, Florida, United States
2 Medicine, University of Florida, Gainesville, Florida, United States
3 Research Service, North Florida/South Georgia Veterans Health System, Gainesville, Florida, United States
4 United States; Medicine, University of Florida, Gainesville, Florida, United States
5 College of Medicine, University of Florida, Gainesville, Florida, United States
6 Department of Biochemistry and Molecular, Biology, Gainsville, Florida, United States; College of Medicine, University of FL, Gainesville, Florida, United States
7 Div. of Nephrol., Hypertension & Transplant., Univ. of Fla. College of Medicine, Gainsville, Florida, United States
8 Renal Division, Dept of Medicine, Emory University, Atlanta, Georgia, United States
9 Research Service, North Florida/South Georgia Veterans Health System, Gainesville, Florida, United States; Medicine, University of Florida, Gainesville, Florida, United States
* To whom correspondence should be addressed. E-mail: cswingo{at}ufl.edu.
Mechanisms of K+ secretion and absorption along the collecting duct are not understood fully. Because KCNQ1 participates in K+ secretion within the inner ear and stomach, the distribution of KCNQ1 in mouse kidney was studied using Northern and Western analyses, RT-PCR of isolated tubules, and immunohistochemistry. Northern blots demonstrated KCNQ1 transcripts in whole kidney. RT-PCR showed KCNQ1 mRNA in isolated distal convoluted tubule (DCT), connecting segment (CNT), collecting ducts (CD), and glomeruli. Immunoblots of kidney and stomach revealed a ~75 kDa protein, the expected mobility for KCNQ1. KCNQ1 was detected by immunohistochemistry throughout the distal nephron and CD. Thick ascending limbs exhibited weak basolateral immunolabel. In DCT and CNT cells, immunolabel was intense and basolateral, although KCNQ1 label was stronger in late DCT than in early DCT. Initial collecting tubule and cortical CD KCNQ1 immunolabel was predominantly diffuse, but many cells had discrete apical label. Double labeling experiments demonstrated that principal cells, type B intercalated cells, and occasional type A intercalated cells exhibited distinct apical KCNQ1 immunolabel. In inner medullary CD, principal cells had distinct basolateral KCNQ1 immunolabel whereas intercalated cells had diffuse cytoplasmic staining. Thus, KCNQ1 protein is widely distributed in mouse distal nephron and collecting duct, with significant axial and cellular heterogeneity in location and intensity. These findings suggest that KCNQ1 has cell-specific roles in renal ion transport and may participate in the process of K+ secretion and/or absorption along the thick ascending limb, distal convoluted tubule, connecting tubule and collecting duct.
This article has been cited by other articles:
|
|
 |
|
  H.-Y. Kim, C. Baylis, J. W. Verlander, K.-H. Han, S. Reungjui, M. E. Handlogten, and I. D. Weiner Effect of reduced renal mass on renal ammonia transporter family, Rh C glycoprotein and Rh B glycoprotein, expression Am J Physiol Renal Physiol, October 1, 2007; 293(4): F1238 - F1247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. vanTol, S. Missan, J. Crack, S. Moser, W. H. Baldridge, P. Linsdell, and E. A. Cowley Contribution of KCNQ1 to the regulatory volume decrease in the human mammary epithelial cell line MCF-7 Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1010 - C1019. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
