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1 Department of Pharmacology, National Institute for Child Health and Development, Tokyo, Tokyo, Japan
2 Department of Nephrology, KumamotoUniversity GraduateSchool of Medical Sciences, Kumamoto, Kumamoto, Japan
3 Department of Pharmacology, National Institute for Child Health and Develpment, Tokyo, Japan
4 Department of Pharmacy, Kumamoto University Hospital, United States
5 Department of Physiology, Kitasato University School of Medicine, Kanagawa, Japan
6 kumamoto, Kumamoto, Japan; Department of Nephrology, KumamotoUniversity GraduateSchool of Medical Sciences, Kumamoto, Kumamoto, Japan
7 Tokyo, Japan; Department of Pharmacology, National Institute for Child Health and Develpment, Tokyo, Japan
8 Department of Physiology, Kitasato University School of Medicine, United States
* To whom correspondence should be addressed. E-mail: nono{at}kumamoto-u.ac.jp.
The neuropeptide hormone arginine-vasopressin (AVP) is well known to exert its antidiuretic effect via the vasopressin V2 receptor (V2R), whereas the role of vasopressin V1a receptor (V1aR) in the kidney remains to be clarified. Previously, we reported decreased plasma volume and blood pressure in V1a receptor-deficient (V1aR-/-) mice. In this study, we investigated the role of V1aR in urine concentration, renal function, and the renin-angiotensin system (RAS) using V1aR-/- mice. Urine volume of V1aR-/- mice was greater than that of wild-type (WT) mice, particularly when water was loaded, while the glomerular filtration rate (GFR), urinary NaCl excretion, AVP-dependent cAMP generation, V2R and aquaporin 2 (AQP2) expression in the kidney were lower, indicating that the diminished GFR and V2R-AQP2 system led to impaired urinary concentration in V1aR-/- mice. Since the GFR and V2R-AQP2 system are regulated by RAS, we analyzed renin and angiotensin II in V1aR-/- mice and found that the plasma renin and angiotensin II were decreased. The expression of renin in granule cells was decreased in V1aR-/- mice, which led to a decreased level of plasma renin. In addition, the expression of renin stimulators such as neuronal nitric oxide synthase (nNOS) and cyclooxygenase (COX)-2 in macula densa (MD) cells, where V1aR was specifically expressed, was decreased in V1aR-/- mice. These data indicate that AVP regulates body fluid homeostasis and GFR via the V1aR in MD cells by activating RAS and subsequently the V2R-AQP2 system.
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