| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Articles in PresS, published online ahead of print July 16, 2002
Am J Physiol Renal Physiol, 10.1152/ajprenal.00102.2002
Submitted on March 15, 2002
Accepted on July 13, 2002
1 Division of Hypertension and Vascular Research, Henry Ford Health System, Detroit, MI, USA
* To whom correspondence should be addressed. E-mail: jgarvin1{at}hfhs.org.
The thick ascending limb (THAL) plays an important role in the regulation of NaCl and water reabsorption. In vivo studies have shown that the free radical superoxide (O2-) stimulates Na and water reabsorption by the kidney. However, it is not known whether O2- regulates transport along the nephron in general or in the THAL specifically. We hypothesized that O2- stimulates THAL NaCl reabsorption. Cl absorption was measured in isolated, perfused THALs from Sprague-Dawley rats. First we tested whether extracellular O2- stimulates Cl absorption. Addition of the O2- generating system xanthine oxidase/hypoxanthine increased Cl absorption from 112.7 ± 12.0 to 146.2 ± 13.9 pmol/mm/min, a 30% increase (p < 0.03). When superoxide dismutase (300 U/ml) was present in the bath, addition of xanthine oxidase/hypoxanthine did not significantly increase Cl absorption (116.9 ± 13.8 vs. 102.5 ± 8.5 pmol/mm/min). Furthermore, adding 200 nM H2O2 to the bath did not significantly affect Cl absorption (from 130.3 ± 13.7 to 125.3 ± 19.6 pmol/mm/min). Since extracellular O2- stimulated Cl absorption, we next tested whether endogenously produced O2- could stimulate transport. Under basal conditions, THALs produced detectable amounts of O2- as measured by lucigenin-enhanced chemiluminescence. Adding the O2- scavenger Tempol to the bath decreased Cl absorption from 198.1 ± 35.4 to 132.4 ± 23.5 pmol/mm/min, a 31% decrease ( p < 0.02). To make sure Tempol was not exerting cytotoxic effects, we tested whether its effect was reversible. With Tempol in the bath, Cl absorption was 117.2 ± 9.3 pmol/mm/min. Sixty minutes after Tempol was removed from the bath, Cl absorption increased to 149.2 ± 9.1 pmol/mm/min (p < 0.05). We conclude that both exogenous and endogenous O2- stimulate THAL NaCl absorption. To our knowledge, these are the first data showing a direct effect of O2- on nephron transport.
This article has been cited by other articles:
|
|
 |
|
  G. B. Silva and J. L. Garvin Angiotensin II-Dependent Hypertension Increases Na Transport-Related Oxygen Consumption by the Thick Ascending Limb Hypertension, December 1, 2008; 52(6): 1091 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kelsen, B. J. Patel, L. B. Parker, T. Vera, J. M. Rimoldi, R. S. V. Gadepalli, H. A. Drummond, and D. E. Stec Heme oxygenase attenuates angiotensin II-mediated superoxide production in cultured mouse thick ascending loop of Henle cells Am J Physiol Renal Physiol, October 1, 2008; 295(4): F1158 - F1165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Vera, S. Kelsen, and D. E. Stec Kidney-Specific Induction of Heme Oxygenase-1 Prevents Angiotensin II Hypertension Hypertension, October 1, 2008; 52(4): 660 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. G. Abraham Gene Targeting and Heme Oxygenase-1 Expression in Prevention of Hypertension Induced by Angiotensin II Hypertension, October 1, 2008; 52(4): 618 - 620. [Full Text] [PDF]
|
|
|
|
|
|
M. Z. Haque and D. S. A. Majid Reduced renal responses to nitric oxide synthase inhibition in mice lacking the gene for gp91phox subunit of NAD(P)H oxidase Am J Physiol Renal Physiol, September 1, 2008; 295(3): F758 - F764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Moreno, I. Rodriguez Gomez, R. Wangensteen, M. Alvarez-Guerra, J. d. D. Luna, J. Garcia-Estan, and F. Vargas Tempol improves renal hemodynamics and pressure natriuresis in hyperthyroid rats Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R867 - R873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Stec, H. A. Drummond, and T. Vera Role of Carbon Monoxide in Blood Pressure Regulation Hypertension, March 1, 2008; 51(3): 597 - 604. [Full Text] [PDF]
|
|
|
|
|
|
J. L. Garvin and N. J. Hong Cellular Stretch Increases Superoxide Production in the Thick Ascending Limb Hypertension, February 1, 2008; 51(2): 488 - 493. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Soodvilai, Z. Jia, and T. Yang Hydrogen peroxide stimulates chloride secretion in primary inner medullary collecting duct cells via mPGES-1-derived PGE2 Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1571 - F1576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Silva and P. Soares-da-Silva Reactive oxygen species and the regulation of renal Na+-K+-ATPase in opossum kidney cells Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2007; 293(4): R1764 - R1770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Vera, S. Kelsen, L. L. Yanes, J. F. Reckelhoff, and D. E. Stec HO-1 induction lowers blood pressure and superoxide production in the renal medulla of angiotensin II hypertensive mice Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2007; 292(4): R1472 - R1478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Just, A. J. M. Olson, C. L. Whitten, and W. J. Arendshorst Superoxide mediates acute renal vasoconstriction produced by angiotensin II and catecholamines by a mechanism independent of nitric oxide Am J Physiol Heart Circ Physiol, January 1, 2007; 292(1): H83 - H92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. Silva, P. A. Ortiz, N. J. Hong, and J. L. Garvin Superoxide Stimulates NaCl Absorption in the Thick Ascending Limb Via Activation of Protein Kinase C Hypertension, September 1, 2006; 48(3): 467 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Schluter, R. Grimm, A. Steinbach, G. Lorenz, R. Rettig, and O. Grisk Neonatal sympathectomy reduces NADPH oxidase activity and vascular resistance in spontaneously hypertensive rat kidneys Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2006; 291(2): R391 - R399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Abe, P. O'Connor, M. Kaldunski, M. Liang, R. J. Roman, and A. W. Cowley Jr. Effect of sodium delivery on superoxide and nitric oxide in the medullary thick ascending limb Am J Physiol Renal Physiol, August 1, 2006; 291(2): F350 - F357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Neuhofer and F.-X. Beck Survival in Hostile Environments: Strategies of Renal Medullary Cells Physiology, June 1, 2006; 21(3): 171 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. K. Dutta, J. Lane, L. J. Roberts II, and D. S. A. Majid Superoxide formation and interaction with nitric oxide modulate systemic arterial pressure and renal function in salt-depleted dogs. Experimental Biology and Medicine, March 1, 2006; 231(3): 269 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. A. Ortiz cAMP increases surface expression of NKCC2 in rat thick ascending limbs: role of VAMP Am J Physiol Renal Physiol, March 1, 2006; 290(3): F608 - F616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kopkan and D. S.A. Majid Enhanced Superoxide Activity Modulates Renal Function in NO-Deficient Hypertensive Rats Hypertension, March 1, 2006; 47(3): 568 - 572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Juncos, N. J. Hong, and J. L. Garvin Differential effects of superoxide on luminal and basolateral Na+/H+ exchange in the thick ascending limb Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R79 - R83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Patel, Y. Chen, K. Dennehy, J. Blau, S. Connors, M. Mendonca, M. Tarpey, M. Krishna, J. B. Mitchell, W. J. Welch, et al. Acute antihypertensive action of nitroxides in the spontaneously hypertensive rat Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2006; 290(1): R37 - R43. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kopkan, A. Castillo, L. G. Navar, and D. S. A. Majid Enhanced superoxide generation modulates renal function in ANG II-induced hypertensive rats Am J Physiol Renal Physiol, January 1, 2006; 290(1): F80 - F86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. S. Wilcox Oxidative stress and nitric oxide deficiency in the kidney: a critical link to hypertension? Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2005; 289(4): R913 - R935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Capasso, M. Rizzo, C. Evangelista, P. Ferrari, G. Geelen, F. Lang, and G. Bianchi Altered expression of renal apical plasma membrane Na+ transporters in the early phase of genetic hypertension Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1173 - F1182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Markadieu, R. Crutzen, D. Blero, C. Erneux, and R. Beauwens Hydrogen peroxide and epidermal growth factor activate phosphatidylinositol 3-kinase and increase sodium transport in A6 cell monolayers Am J Physiol Renal Physiol, June 1, 2005; 288(6): F1201 - F1212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Juncos and J. L. Garvin Superoxide enhances Na-K-2Cl cotransporter activity in the thick ascending limb Am J Physiol Renal Physiol, May 1, 2005; 288(5): F982 - F987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. A. Majid, A. Nishiyama, K. E. Jackson, and A. Castillo Superoxide scavenging attenuates renal responses to ANG II during nitric oxide synthase inhibition in anesthetized dogs Am J Physiol Renal Physiol, February 1, 2005; 288(2): F412 - F419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, K. Rhinehart, G. Solis, J. Pittner, W. Lee-Kwon, W. J. Welch, C. S. Wilcox, and T. L. Pallone Chronic ANG II infusion increases NO generation by rat descending vasa recta Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H29 - H36. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Lee, J. M. Sasser, J. L. Hobbs, A. Boriskie, D. M. Pollock, P. K. Carmines, and J. S. Pollock Posttranslational regulation of NO synthase activity in the renal medulla of diabetic rats Am J Physiol Renal Physiol, January 1, 2005; 288(1): F82 - F90. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zhang, K. Rhinehart, W. Kwon, E. Weinman, and T. L. Pallone ANG II signaling in vasa recta pericytes by PKC and reactive oxygen species Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H773 - H781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. A. Majid, A. Nishiyama, K. E. Jackson, and A. Castillo Inhibition of nitric oxide synthase enhances superoxide activity in canine kidney Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2004; 287(1): R27 - R32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Evans, R. E. Rose, J. M. Roeser, and J. D. Stidham NaCl transport across the opercular epithelium of Fundulus heteroclitus is inhibited by an endothelin to NO, superoxide, and prostanoid signaling axis Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2004; 286(3): R560 - R568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Mori and A. W. Cowley Jr. Renal Oxidative Stress in Medullary Thick Ascending Limbs Produced by Elevated NaCl and Glucose Hypertension, February 1, 2004; 43(2): 341 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Z. Haque and D. S. A. Majid Assessment of Renal Functional Phenotype in Mice Lacking gp91PHOX Subunit of NAD(P)H Oxidase Hypertension, February 1, 2004; 43(2): 335 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zimpelmann, N. Li, and K. D. Burns Nitric oxide inhibits superoxide-stimulated urea permeability in the rat inner medullary collecting duct Am J Physiol Renal Physiol, December 1, 2003; 285(6): F1160 - F1167. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
