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Am J Physiol Renal Physiol (September 23, 2003). doi:10.1152/ajprenal.00113.2003
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Submitted on March 21, 2003
Accepted on September 11, 2003

ANGIOTENSIN II-DEPENDENT INCREASED EXPRESSION OF Na+-GLUCOSE COTRANSPORTER IN HYPERTENSION

Rocio Bautista1, Rebeca Manning1, Flavio Martinez2, Maria del Carmen Avila-casado3, Virgilia Soto3, Armando Medina3, and Bruno Escalante1*

1 Department of Molecular Biomedicine, Centro de Investigacion y Estudios Avanzados del IPN, DF, N/A, Mexico
2 Department of Pharmacology, UASLP, San Luis Potosi, san Luis Potosi, Mexico
3 Department of Pathology, Instituto Nacional de Cardiologia Ingacio Chavez, DF, N/A, Mexico

* To whom correspondence should be addressed. E-mail: bescalan{at}mail.cinvestav.mx.

Glucose uptake is increased in hypertension. Thus, we investigated the Na+-glucose cotransporter (SGLT2) activity and expression in proximal tubules from renovascular hypertensive rats. Sham-operated rats, aortic coarctation rats and aortic coarctation rats treated with either ramipril (2.5 mg/kg/day/21 days) or losartan (10 mg/kg/day/21 days) were used. Na+-dependent glucose uptake was measured in brush border membrane vesicles (BBMV). Vmax value in BBMV of hypertensive rats was greater compared to that of normotensive rats (3±0.2 vs 1.5±0.1 nmol/mg protein/min) without changes in Km. Renal immunostaining, western blot and RT-PCR for SGLT2 in hypertensive rats were higher than normotensive rats (1029±71 vs 5003±92, 199±15 vs 95±10 and 1.4±0.2 vs 0.3±0.1 arbitrary units respectively). In the rats treated with either ramipril or losartan Vmax decreased to 2.1 ± 0.3 and 1.8 ± 0.4 nmol/mg protein/min respectively, as well as the intensity of immunostaining, protein and mRNA levels. We suggests, that in renovascular hypertension, Angiotensin II induced SGLT2 via AT1 receptor, which was evidenced at both functional and expression levels, probably contributing to increased absorption of Na+ thereby to the development or maintenance of hypertension.




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