| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Nephrology, University Hospital of Lund, Lund, Sweden
* To whom correspondence should be addressed. E-mail: catarina{at}rippe.se.
This study was performed to evaluate the alterations of the glomerular filtration barrier characteristics following acute renal ischemia/reperfusion (I/R). Ischemia was induced in anesthetized rats by unilateral renal artery occlusion for either 20 or 60 minutes, followed by reperfusion during 20 or 60 minutes, respectively, with the contralateral kidney serving as control. Sieving coefficients (
) were obtained by analyzing Ficoll [mol.radius (ae) 1.3-8.5 nm] in urine and plasma after 20 and 60 min I/R. Furthermore, for human serum albumin (HSA) was estimated using a tissue uptake technique after 20 and 60 min of I/R, while clearance of HSA compared to that for neutralized HSA (nHSA) was assessed after 20 min of I/R only. Glomerular filtration rate (GFR) was measured by 51Cr-EDTA and inulin. I/R reduced GFR and increased for Ficoll molecules of ae >5.5 nm and for albumin. for Ficoll vs. ae, analysed using a two-pore model, demonstrated that, despite increases in , the large pore fractional ultrafiltration coefficient (
L) was unchanged after 20 min of I/R, owing to the decline in GFR, but increased after 60 min of I/R. However, the apparent L for albumin, increased already after 20 min of I/R (p<0.005) and the nHSA/HSA clearance ratio was slightly reduced, possibly reflecting a diminished negative charge barrier. In conclusion, after 20 min of I/R indications of a reduced charge selectivity were noted, while after 60 min of I/R, there was mainly a reduction in size selectivity, compatible with an increased formation of large pores.
This article has been cited by other articles:
|
|
 |
|
  A. V. Cybulsky, T. Takano, J. Guillemette, J. Papillon, R. A. Volpini, and J. A. Di Battista The Ste20-like kinase SLK promotes p53 transactivation and apoptosis Am J Physiol Renal Physiol, October 1, 2009; 297(4): F971 - F980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Axelsson, I. Mahmutovic, A. Rippe, and B. Rippe Loss of size selectivity of the glomerular filtration barrier in rats following laparotomy and muscle trauma Am J Physiol Renal Physiol, September 1, 2009; 297(3): F577 - F582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Grande, C. Rippe, A. Rippe, A. Rahman, K. Sward, and B. Rippe Unaltered size selectivity of the glomerular filtration barrier in caveolin-1 knockout mice Am J Physiol Renal Physiol, August 1, 2009; 297(2): F257 - F262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Tanner, C. Rippe, Y. Shao, A. P. Evan, and J. C. Williams Jr. Glomerular permeability to macromolecules in the Necturus kidney Am J Physiol Renal Physiol, June 1, 2009; 296(6): F1269 - F1278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Axelsson, A. Rippe, D. Venturoli, P. Sward, and B. Rippe Effects of early endotoxemia and dextran-induced anaphylaxis on the size selectivity of the glomerular filtration barrier in rats Am J Physiol Renal Physiol, February 1, 2009; 296(2): F242 - F248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Rippe Free water transport, small pore transport and the osmotic pressure gradient three-pore model of peritoneal transport Nephrol. Dial. Transplant., July 1, 2008; 23(7): 2147 - 2153. [Full Text] [PDF]
|
|
|
|
|
|
C. Rippe, A. Rippe, O. Torffvit, and B. Rippe Size and charge selectivity of the glomerular filter in early experimental diabetes in rats Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1533 - F1538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Andersson, U. Nilsson, C. Hjalmarsson, B. Haraldsson, and J. S. Nystrom Mild renal ischemia-reperfusion reduces charge and size selectivity of the glomerular barrier Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1802 - F1809. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
