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1 Department of Obstetrics and Gynecology, Chung-Shan Medical University Hospital, Taichung, Taiwan - Republic of China
2 Department of Veterinary Medicine, National Chung-Hsing Unviersity, Taichung, Taiwan - Republic of China; Department of Physiology, College of Medicine, Chung-Shan Medical University, Taichung, Taiwan - Republic of China
3 Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan - Republic of China
4 division of Urology, Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan - Republic of China
5 Department of Nursing, Cardinal Tien College of Healthcare and Management, Taipei, Taiwan - Republic of China; Department of Pharmacy, St. Paul, Taoyuan, Taiwan - Republic of China
6 Department of Physiology, College of Medicine, Chung-Shan Medical University, Taichung, Taiwan - Republic of China
7 Department of Biotechnology, Ming-Chuan University, Taoyuan, Taiwan - Republic of China
8 Department of Biomedical Engineering, Ming-Chuan University, Taoyuan, Taiwan - Republic of China
9 Department of Physiology, College of Medicine, Chung-Shan Medical University, Taichung, Taiwan - Republic of China; Department of Medicine, St. Paul, Taichung, Taiwan - Republic of China
* To whom correspondence should be addressed. E-mail: tblin{at}csmu.edu.tw.
This study was conducted to investigate whether dorsolateral pontine tegmentum stimulation modulates spinal reflex potentiation (SRP) and whether serotonergic neurotransmission is involved in such a modulation. Reflex activities of the external urethra sphincter electromyogram in response to a test stimulation (TS, 1/30 Hz) or repetitive stimulation (RS, 1 Hz) on the pelvic afferent nerve in 35 anesthetized rats were recorded with/without synchronized train pontine stimulation (PS, 300 Hz, 30 ms) and/or intrathecal administrations of 10 µL of 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo (F) quinoxaline (NBQX, 100 µM), D-2-amino-5-phosphonovalerate (APV, 100µM), N-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-N-(2-pyridinyl) cyclohexanecarboxamide trihydrochloride (WAY100635, 100 µM) and 8-hydroxy-2-(di-n-propylamino)-tetralin (8-OH-DPAT, 100 µM). The TS evoked a single action potential (1.00±0.00 spikes/stimulation), while the RS produced a long-lasting SRP (16.12±1.59 spikes/stimulation) that was abolished by APV (1.57±0.29 spikes/stimulation) and was attenuated by NBQX (7.42±0.57 spikes/stimulation). Synchronized train pontine stimulation with RS (PS+RS) produced facilitation in RS-induced SRP (25.17±2.21 spikes/stimulation). Intrathecal WAY 100635 abolished the facilitation in SRP as a result of the synchronized pontine stimulation (14.66±1.58 spikes/stimulation). On the other hand, intrathecal 8-OH-DPAT elicited facilitation in the RS-induced SRP (25.16±1.05 spikes/stimulation) without synchronized pontine stimulation. Our findings suggest that dorsolateral pontine tegmentum may modulate NMDA-dependent SRP via descending serotonergic neurotransmission. This descending modulation may have physiological/pharmacological relevance in the neural controls of urethral closure.
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