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Articles in PresS, published online ahead of print January 28, 2002
Am J Physiol Renal Physiol, 10.1152/ajprenal.00136.2001
Submitted on May 1, 2001
Accepted on January 2, 2002
1 Division of Renal Medicine, Johns Hopkins Bayview Medical Center, Baltimore, MD, USA
2 Departmen of Physiology, Johns Hopkins Bayview Medical Center, Baltimore, MD, USA
3 Department of Medicine, Johns Hopkins Bayview Medical Center, Baltimore, MD, USA
4 Department of Medicine, Johns Hopkins Bayview Medical Center, Baltimore, MD, USA; Department of Veterans Affairs, University of Maryland School of Medicine, Baltimore, MD, USA
Although mammalian urothelia are generally considered impermeable to constituents of urine, in-vivo studies in several species indicate urothelial transport of water and solutes under certain conditions. This study investigates the expression, localization, and regulation of aquaporins 1, 2, and 3 in ureter and bladder tissues in 48 hour dehydrated and water-loaded female Wistar rats. Immunoblots of homogenates of whole ureter and bladder identified characteristic ~28 and 35-44 kDa bands for aquaporin 1, 2, and 3. Aquaporin 1 was localized to capillary and arteriole endothelial cells, while aquaporin 2 and 3 circumferentially lined the epithelial cell membranes except for the apical membrane of the epithelial cells adjacent to the lumens of both ureter and bladder. Aquaporin 2 also was present in epithelial cell cytoplasm. Dehydration resulted in 160-200% increases of AQP3 signal, 24-49% increases of AQP2 signal, but no change in AQP1 signal on immunoblots of homogenates of ureters and bladders. Aquaporins in GU tract urothelia likely play a role in regulation of epithelia cell volume and osmolality and may play a role in bulk water movement across urothelia.
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