| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Anatomy, The Catholic University of Korea, Seoul, Maryland, Korea, Republic of
2 Anatomy, The Catholic University of Korea, Seoul, Korea, Republic of
3 Internal Medicine, KangNam St. Mary's Hospital, Seoul, Korea, Republic of
4 Anatomy, Ewha Womans University, Seoul, Korea, Republic of
5 Medicine, University of Maryland, Baltimore, Maryland, United States
* To whom correspondence should be addressed. E-mail: jinkim{at}catholic.ac.kr.
This study was to test the hypothesis that, during the renal development, the Na-K-2Cl cotransporter (NKCC2) activates the TonEBP transcription factor by creating medullary hypertonicity. TonEBP in turn drives the expression of aldose reductase (AR) and urea transporter-A (UT-A). Kidneys from 13- to19-day-old fetuses (F13-F19), 1- to 21-day-old pups (P1-P21), and adult mice were examined by immunohistochemistry. NKCC2 was first detected on F14 in differentiating macula densa and thick ascending limb (TAL). TonEBP was first detected on F15 in the medullary collecting duct (MCD) and surrounding of endothelial cells. AR was detected in the MCD cells of renal medulla from F15. UT-A first appeared in the descending thin limb (DTL) on F16 and in the MCD on F18. After birth, NKCC2-positive TALs disappeared gradually from the tip of the renal papilla, becoming completely undetectable in the inner medulla on P21. TonEBP shifted from the cytoplasm to the nucleus in both vascular endothelial cells and MCD cells on P1, and its abundance increased gradually afterwards. Immunoreactivity for AR and UT-A in the renal medulla increased markedly after birth. Treatment of neonatal animals with furosemide dramatically reduced expression of TonEBP, AR, and UT-A1. Furosemide also prevented the disappearance of NKCC2-expressing TALs in the papilla. The sequential expression of NKCC2, TonEBP, and its targets AR and UT-A, and the reduced expression TonEBP and its targets in response to furosemide treatment support the hypothesis that local hypertonicity produced by the activity of NKCC2 activates TonEBP during development.
This article has been cited by other articles:
|
|
 |
|
  M. S. Kwon, S. W. Lim, and H. M. Kwon Hypertonic Stress in the Kidney: A Necessary Evil Physiology, June 1, 2009; 24(3): 186 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hao, H. Zhao, Z. Darzynkiewicz, S. Battula, and N. R. Ferreri Expression and function of NFAT5 in medullary thick ascending limb (mTAL) cells Am J Physiol Renal Physiol, June 1, 2009; 296(6): F1494 - F1503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-M. Kim, W.-Y. Kim, H.-W. Lee, J. Kim, H. M. Kwon, J. D. Klein, J. M. Sands, and D. Kim Urea and NaCl regulate UT-A1 urea transporter in opposing directions via TonEBP pathway during osmotic diuresis Am J Physiol Renal Physiol, January 1, 2009; 296(1): F67 - F77. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Dilworth, M. J. Clancy, D. Marshall, C. A. Bravery, P. E. Brenchley, and N. Ashton Development and functional capacity of transplanted rat metanephroi Nephrol. Dial. Transplant., March 1, 2008; 23(3): 871 - 879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. S. Jeon, K.-H. Han, S.-H. Park, S. D. Lee, M. R. Sheen, J.-Y. Jung, W. Y. Kim, J. M. Sands, J. Kim, and H. M. Kwon Downregulation of renal TonEBP in hypokalemic rats Am J Physiol Renal Physiol, July 1, 2007; 293(1): F408 - F415. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
