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Articles in PresS, published online ahead of print September 21, 2001
Am J Physiol Renal Physiol, 10.1152/ajprenal.00146.2001
Submitted on May 10, 2001
Accepted on September 13, 2001
subunit in rat kidney
1 Neuroscience Center, Massachusetts General Hospital, Charlestown, MA, USA
* To whom correspondence should be addressed. E-mail: sweadner{at}helix.mgh.harvard.edu.
Renal Na,K-ATPase is associated with the subunit (FXYD2), a single-span membrane protein that modifies ATPase properties. There are two splice variants with different N-termini, a and b. Both were found in the inner stripe of the outer medulla in thick ascending limb. Coimmunoprecipitation with each other and 
indicated that they were associated in macromolecular complexes. Association was controlled by ligands that affect Na,K-ATPase conformation. In cortex, the proportion of b was markedly lower, and a predominated in isolated proximal tubule cells. By immunofluorescence, b was detected in superficial cortex only in distal convoluted tubule and connecting tubule, which are rich in Na,K-ATPase but comprise a minor fraction of cortex mass. In the outer stripe of outer medulla, and for a short distance in deep cortex, thick ascending limb expressed predominantly b. Since different mechanisms maintain and regulate sodium homeostasis in different nephron segments, the splice forms of may have evolved to control renal sodium pump through pump properties, gene expression, or both.
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