AJP - Renal Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Renal Physiol (July 18, 2006). doi:10.1152/ajprenal.00177.2006
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
291/6/F1184    most recent
00177.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jernigan, N. L
Right arrow Articles by Drummond, H. A
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jernigan, N. L
Right arrow Articles by Drummond, H. A
Submitted on May 23, 2006
Accepted on July 12, 2006

Myogenic Vasoconstriction in Mouse Renal Interlobar Arteries: Role of Endogenous {beta} and {gamma}ENaC

Nikki L Jernigan1 and Heather A Drummond1*

1 Physiology and Biophysics, University of Mississippi Medical Center, Jackson, Mississippi, United States

* To whom correspondence should be addressed. E-mail: hdrummond{at}physiology.umsmed.edu.

Mechanosensitive ion channels are thought to initiate pressure-induced vasoconstriction, however the molecular identity of these channels is unknown. Recent work from our laboratory suggests that members of the Degenerin/Epithelial Na+ Channel (DEG/ENaC) family may be components of the mechanosensitive ion channel complex in vascular smooth muscle; however the specific DEG/ENaC proteins mediating myogenic constriction are unknown. The goal of this study is to determine if specific knockdown of {beta} or {gamma}ENaC, using dominant-negative (DN) or small interfering RNA (siRNA) molecules, inhibits pressure-induced vasoconstriction in mouse renal interlobar arteries. To address this goal, isolated arteries were transiently transfected with {beta}or {gamma}ENaC DN-cDNA or siRNA molecules. Following 24 hrs, vessels were either 1) cannulated and pressurized for pressure-diameter response curves or 2) dissociated and immunolabeled to determine VSM cell endogenous ENaC protein expression. We found that transfection of {beta}ENaC DN-cDNA or siRNA suppresses {beta}-, but not {gamma}ENaC protein expression. Similarly, {gamma}ENaC DN-cDNA or siRNA suppresses {gamma}-, but not {beta}ENaC protein expression. In addition, transfection of {beta}- or {gamma}ENaC DN-cDNA or siRNA molecules inhibits pressure-induced vasoconstriction, but does not block agonist-induced vasoconstriction. Our results provide the first direct evidence that {beta}and {gamma}ENaC proteins are essential in mediating myogenic vasoconstriction in mouse renal interlobar arteries.




This article has been cited by other articles:


Home page
 HypertensionHome page
Z. Guan, J. S. Pollock, A. K. Cook, J. L. Hobbs, and E. W. Inscho
Effect of Epithelial Sodium Channel Blockade on the Myogenic Response of Rat Juxtamedullary Afferent Arterioles
Hypertension, November 1, 2009; 54(5): 1062 - 1069.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Kapoor, R. Bartoszewski, Y. J. Qadri, Z. Bebok, J. K. Bubien, C. M. Fuller, and D. J. Benos
Knockdown of ASIC1 and Epithelial Sodium Channel Subunits Inhibits Glioblastoma Whole Cell Current and Cell Migration
J. Biol. Chem., September 4, 2009; 284(36): 24526 - 24541.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
L. G. VanLandingham, K. P. Gannon, and H. A. Drummond
Pressure-induced constriction is inhibited in a mouse model of reduced {beta}ENaC
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2009; 297(3): R723 - R728.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
N. L. Jernigan, M. L. Paffett, B. R. Walker, and T. C. Resta
ASIC1 contributes to pulmonary vascular smooth muscle store-operated Ca2+ entry
Am J Physiol Lung Cell Mol Physiol, August 1, 2009; 297(2): L271 - L285.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
F. R. Perez, F. Venegas, M. Gonzalez, S. Andres, C. Vallejos, G. Riquelme, J. Sierralta, and L. Michea
Endothelial Epithelial Sodium Channel Inhibition Activates Endothelial Nitric Oxide Synthase via Phosphoinositide 3-Kinase/Akt in Small-Diameter Mesenteric Arteries
Hypertension, June 1, 2009; 53(6): 1000 - 1007.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. Ponnuchamy and R. A. Khalil
Cellular mediators of renal vascular dysfunction in hypertension
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2009; 296(4): R1001 - R1018.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
B. L. Hodnett, J. A. Dearman, C. B. Carter, and R. L. Hester
Attenuated PGI2 synthesis in obese Zucker rats
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2009; 296(3): R715 - R721.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
E. W. Inscho
Mysteries of Renal Autoregulation
Hypertension, February 1, 2009; 53(2): 299 - 306.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. L. McCord, S. G. Hayes, and M. P. Kaufman
Acid-sensing ion and epithelial sodium channels do not contribute to the mechanoreceptor component of the exercise pressor reflex
Am J Physiol Heart Circ Physiol, September 1, 2008; 295(3): H1017 - H1024.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
X. Wang, K. Takeya, P. I. Aaronson, K. Loutzenhiser, and R. Loutzenhiser
Effects of amiloride, benzamil, and alterations in extracellular Na+ on the rat afferent arteriole and its myogenic response
Am J Physiol Renal Physiol, July 1, 2008; 295(1): F272 - F282.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. C. Grifoni, S. E. McKey, and H. A. Drummond
Hsc70 regulates cell surface ASIC2 expression and vascular smooth muscle cell migration
Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2022 - H2030.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
H. A. Drummond, N. L. Jernigan, and S. C. Grifoni
Sensing Tension: Epithelial Sodium Channel/Acid-Sensing Ion Channel Proteins in Cardiovascular Homeostasis
Hypertension, May 1, 2008; 51(5): 1265 - 1271.
[Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
K. P. Gannon, L. G. VanLandingham, N. L. Jernigan, S. C. Grifoni, G. Hamilton, and H. A. Drummond
Impaired pressure-induced constriction in mouse middle cerebral arteries of ASIC2 knockout mice
Am J Physiol Heart Circ Physiol, April 1, 2008; 294(4): H1793 - H1803.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
H. A. Drummond, S. C. Grifoni, and N. L. Jernigan
A New Trick for an Old Dogma: ENaC Proteins as Mechanotransducers in Vascular Smooth Muscle
Physiology, February 1, 2008; 23(1): 23 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
R. Schubert, D. Lidington, and S.-S. Bolz
The emerging role of Ca2+ sensitivity regulation in promoting myogenic vasoconstriction
Cardiovasc Res, January 1, 2008; 77(1): 8 - 18.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
N. L. Jernigan, B. LaMarca, J. Speed, L. Galmiche, J. P. Granger, and H. A. Drummond
Dietary salt enhances benzamil-sensitive component of myogenic constriction in mesenteric arteries
Am J Physiol Heart Circ Physiol, January 1, 2008; 294(1): H409 - H420.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
H. A. Coleman and H. C. Parkington
Endothelin enhances activity of mechanosensitive channels: A mechanism for ET augmentation of the myogenic response
Cardiovasc Res, November 1, 2007; 76(2): 197 - 198.
[Full Text] [PDF]

Home page
 HypertensionHome page
C. J. Busst, K. J. Scurrah, J. A. Ellis, and S. B. Harrap
Selective Genotyping Reveals Association Between the Epithelial Sodium Channel {gamma}-Subunit and Systolic Blood Pressure
Hypertension, October 1, 2007; 50(4): 672 - 678.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 1977 by the American Physiological Society.