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Am J Physiol Renal Physiol (August 5, 2003). doi:10.1152/ajprenal.00198.2003
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Submitted on May 27, 2003
Accepted on July 30, 2003

Over-expression of PKC-{beta}I and -{delta} contributes to higher PKC activity in the proximal tubules of old Fischer 344 rats

Mohammad Asghar1, Tahir Hussain1, and Mustafa F. Lokhandwala1*

1 Heart and Kidney Institute, College of Pharmacy, University of Houston, Houston, TX, USA

* To whom correspondence should be addressed. E-mail: MLOKHANDWALA{at}UH.EDU.

Previously, we have reported that natriuretic and diuretic response to dopamine is diminished in old Fischer 344 rats which is due to higher basal protein kinase C (PKC) activity and hyper-phosphorylation of Na,K-ATPase in the proximal tubules (PTs) of old rats. The present study was conducted to determine whether higher PKC activity could be due to altered expression of some of the PKC isoforms in the superficial cortex (rich in PTs) of old rats. Fluorimetric measurement showed almost two-fold increase in the PKC activities in homogenates and membranes of old (24-month) compared to adult (6-month) rats. Interestingly, in the basal state PKC-{beta}I was over-expressed in the membranes while PKC-{delta} expression was increased in the cytosol of old compared to adult rats. Treatment of the cortical slices with either SKF 38393, a D1-like agonist, or PDBu, a direct activator of PKC caused translocation of PKC-{beta}I from cytosol to membranes in adult but not in old rats. Both these drugs caused translocation of PKC-{delta} from membranes to cytosol in adult but not in old rats. These drugs had no effect on translocation of PKC-{zeta} in both adult and old rats. Both PKC-{beta}I and -{delta} co-immunoprecipiated with {alpha}1-subunit of Na,K-ATPase in adult and old rats. These observations suggest that both SKF 38393 and PDBu differentially regulate PKC-{beta}I and -{delta} in adult but not in old rats. Also, PKC-{beta}I and -{delta} seem to interact with Na,K-ATPase in these animals. The over-expression of both PKC-{beta}I and -{delta} in old rats could be responsible for a higher basal PKC activity, which causes the hyper-phosphorylation of Na,K-ATPase and contributes to the diminished inhibition of Na,K-ATPase activity by dopamine in old rats.




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