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1 Department of Medicine, University of Mississippi Medical Center, Jackson, MS, USA
2 Department of Pathology, University of Mississippi Medical Center, Jackson, MS, USA
* To whom correspondence should be addressed. E-mail: mflessner{at}medicine.umsmed.edu.
To study the process of chronic peritoneal inflammation from sterile solutions, we established an animal model to link structural changes with solute and water transport. Filtered solutions containing 4% n-acetyl-glucosamine (NAG) or 4% glucose (G) were injected ip daily in 200-300 g rats and compared with controls (C). After 2 months, each animal underwent transport studies using a chamber affixed to parietal peritoneum to determine: small solute and protein mass transfer, osmotic filtration, and hydraulic flow. After euthanasia, parietal tissues were sampled for histological analysis, which demonstrated significant differences in peritoneal thickness (µm, C, 42.6 ± 7.5; G, 80.4 ± 22.3; NAG, 450 ± 104; p < .05). Staining for VEGF correlated with CD-31 vessel counts (no./mm2: C, 53.1 ± 16.1; G, 166 ± 32; NAG, 183 ± 32; p < .05 ). Tissue analysis showed treatment effects on tissue hyaluronan (µg/g: C, 962 ± 73; G, 1169 ± 69; NAG, 1428 ± 69; p < 0.05) and collagen (µg/g: C, 56.9 ± 12.0; G, 107 ± 12; NAG, 97.6 ± 11.4; p < 0.05) but not sulfated glycosaminoglycan. Transport experiments revealed no significant differences in mannitol transfer or osmotic flow. Changes were seen in hydrostatic pressure driven flux (µl/min/cm2: C, 0.676 ± 0.133; G, 0.317 ± 0.124; NAG, 0.284 ± 0.117; p<.05) and albumin transfer (µl/min/cm2: C, 0.331 ± 0.028; G, 0.286 ± 0.026; NAG, 0.229 ± 0.025; p < .04). We conclude that alteration of the interstitial matrix correlates with diminished hydraulic conductivity and macromolecular transport.
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