| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Medicine, Nephrology, UW Madison, Madison, Wisconsin, United States
2 Radiology, UW Madison, Madison, Wisconsin, United States
3 Surgery, University of Wisconsin-Madison, Madison, Wisconsin, United States
* To whom correspondence should be addressed. E-mail: axd{at}medicine.wisc.edu.
Blood oxygen level-dependent (BOLD) magnetic resonance imaging (MRI) uses deoxyhemoglobin as an endogenous contrast agent for the noninvasive assessment of tissue oxygen bioavailability. We hypothesized that intrarenal oxygenation was impaired in patients with chronic allograft nephropathy (CAN). Ten kidney-transplant recipients with CAN and 9 healthy volunteers underwent BOLD-MRI. Medullary R2* (MR2*) and cortical R2* (CR2*) levels (measures directly proportional to tissue deoxyhemoglobin levels) were determined alongside with urine and serum markers of oxidative stress (OS): hydrogen peroxide (H2O2), F2-isoprostanes, total nitric oxide (NO), heat shock protein 27 (HSP27) and total antioxidant property (TAOP). Mean MR2* and CR2* levels were significantly decreased in CAN (increased local oxyhemoglobin concentration) compared to healthy volunteers (20.7/s± 1.6 vs. 23.1/s± 1.8, p=0.03 and 15.9± 1.9 vs. 13.6/s± 2.3, p=0.05, respectively). There was a significant increase in serum and urine levels of H2O2 and serum HSP27 levels in patients with CAN. Conversely, urine NO levels and TAOP were significantly increased in healthy volunteers. Multiple linear regression analyses showed a significant association between MR2* and CR2* levels and serum/urine biomarkers of OS. BOLD-MRI demonstrated significant changes in medullary and cortical oxygen bioavailability in allografts with CAN. These correlated with serum/urine biomarkers of OS, suggesting an association between intrarenal oxygenation and OS.
This article has been cited by other articles:
|
|
 |
|
  H. Chandarana and V. S. Lee Renal Functional MRI: Are We Ready for Clinical Application? Am. J. Roentgenol., June 1, 2009; 192(6): 1550 - 1557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Evans, B. S. Gardiner, D. W. Smith, and P. M. O'Connor Intrarenal oxygenation: unique challenges and the biophysical basis of homeostasis Am J Physiol Renal Physiol, November 1, 2008; 295(5): F1259 - F1270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. C. Thoeny, T. M. Kessler, S. Simon-Zoula, F. De Keyzer, M. Mohaupt, U. E. Studer, and P. Vermathen Renal Oxygenation Changes during Acute Unilateral Ureteral Obstruction: Assessment with Blood Oxygen Level-Dependent MR Imaging--Initial Experience Radiology, June 1, 2008; 247(3): 754 - 761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Djamali Oxidative stress as a common pathway to chronic tubulointerstitial injury in kidney allografts Am J Physiol Renal Physiol, August 1, 2007; 293(2): F445 - F455. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
