| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Medicine, Shiga University of Medical Science, Otsu, Shiga, Japan
2 Discovery Research Laboratory, Tanabe Seiyaku Co., Ltd.,, Osaka, Osaka, Japan
* To whom correspondence should be addressed. E-mail: haneda{at}belle.shiga-med.ac.jp.
Persistent proteinuria has been indicated to be a major risk factor for the development of tubulointerstitial damage through a process as proinflammatry molecules expression. Among these, monocyte chemoattractant protein-1 (MCP-1) was shown to contribute to the recruitment of immune cells into the renal interstitium in both acute and chronic renal diseases. However, the molecular mechanisms by which proteinuria causes MCP-1 expression in proximal tubular cells have not been fully clarified yet.
In this study, we examined whether albumin overload-induced MCP-1 expression was regulated by MAPK family in mouse proximal tubular cells (mProx). By exposing mProx to delipidated bovine serum albumin (BSA), mRNA and protein expression of MCP-1 was induced in a time-and dose-dependent manner. BSA was able to activate extracellular signal-regulated kinases 1/2 (ERK1/2) and p38 MAPK. MEK inhibitor (U0126) partially suppressed BSA-induced MCP-1 expression and MCP-1 promoter / luciferase reporter activity. U0126 also inhibited an increase in NF-
B and AP-1 DNA binding activity of MCP-1 promoter by protein overload in mProx. In addition, we found that U0126 inhibited BSA-induced NF-B reporter activity and IB-
degradation in mProx.In conclusion, these findings indicate that ERK signaling is involved in BSA induced MCP-1 expression in mProx.
This article has been cited by other articles:
|
|
 |
|
  G. H. Tesch MCP-1/CCL2: a new diagnostic marker and therapeutic target for progressive renal injury in diabetic nephropathy Am J Physiol Renal Physiol, April 1, 2008; 294(4): F697 - F701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Wong, Y. Suzuki, C. Tanifuji, H. Akiba, K. Okumura, T. Sugaya, T. Yamamoto, S. Horikoshi, S. Y. Tan, C. Pollock, et al. Peritubular Ischemia Contributes More to Tubular Damage than Proteinuria in Immune-Mediated Glomerulonephritis J. Am. Soc. Nephrol., February 1, 2008; 19(2): 290 - 297. [Full Text] [PDF]
|
|
|
|
|
|
C. K. Fujihara, D. M. A. C. Malheiros, and R. Zatz Losartan-hydrochlorothiazide association promotes lasting blood pressure normalization and completely arrests long-term renal injury in the 5/6 ablation model Am J Physiol Renal Physiol, June 1, 2007; 292(6): F1810 - F1818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Diwakar, A. L. Pearson, P. Colville-Nash, N. J. Brunskill, and M. E. C. Dockrell The role played by endocytosis in albumin-induced secretion of TGF-beta1 by proximal tubular epithelial cells Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1464 - F1470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Murali, A. W. Ackerman, A. J. Croatt, J. Cheng, J. P. Grande, S. L. Sutor, R. J. Bram, G. D. Bren, A. D. Badley, J. Alam, et al. Renal upregulation of HO-1 reduces albumin-driven MCP-1 production: implications for chronic kidney disease Am J Physiol Renal Physiol, February 1, 2007; 292(2): F837 - F844. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yu, J. Yeh, and C. Van Waes Protein Kinase Casein Kinase 2 Mediates Inhibitor-{kappa}B Kinase and Aberrant Nuclear Factor-{kappa}B Activation by Serum Factor(s) in Head and Neck Squamous Carcinoma Cells. Cancer Res., July 1, 2006; 66(13): 6722 - 6731. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Schlesinger, V. van Harmelen, C. E. Alberti-Huber, and H. Hauner Albumin inhibits adipogenesis and stimulates cytokine release from human adipocytes Am J Physiol Cell Physiol, July 1, 2006; 291(1): C27 - C33. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shibata, M. Nagase, and T. Fujita Fluvastatin Ameliorates Podocyte Injury in Proteinuric Rats via Modulation of Excessive Rho Signaling J. Am. Soc. Nephrol., March 1, 2006; 17(3): 754 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. de Haij, A. C. Bakker, R. N. van der Geest, G. Haegeman, W. Vanden Berghe, J. Aarbiou, M. R. Daha, and C. van Kooten NF-{kappa}B Mediated IL-6 Production by Renal Epithelial Cells Is Regulated by C-Jun NH2-Terminal Kinase J. Am. Soc. Nephrol., June 1, 2005; 16(6): 1603 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Reich, D. Tritchler, A. M. Herzenberg, Z. Kassiri, X. Zhou, W. Gao, and J. W. Scholey Albumin Activates ERK Via EGF Receptor in Human Renal Epithelial Cells J. Am. Soc. Nephrol., May 1, 2005; 16(5): 1266 - 1278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Okada, Y. Watanabe, T. Kikuta, T. Kobayashi, Y. Kanno, T. Sugaya, and H. Suzuki Bradykinin Decreases Plasminogen Activator Inhibitor-1 Expression and Facilitates Matrix Degradation in the Renal Tubulointerstitium under Angiotensin-Converting Enzyme Blockade J. Am. Soc. Nephrol., September 1, 2004; 15(9): 2404 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nakajima, M. Takenaka, J.-Y. Kaimori, T. Hamano, H. Iwatani, T. Sugaya, T. Ito, M. Hori, and E. Imai Activation of the Signal Transducer and Activator of Transcription Signaling Pathway in Renal Proximal Tubular Cells by Albumin J. Am. Soc. Nephrol., February 1, 2004; 15(2): 276 - 285. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
